TY - JOUR T1 - Characterization of “Brachyspira hampsonii” clades I and II isolated from commercial swine in Western Canada AU - Perez, Jason Byron D.S. AU - Rubin, Joseph E. AU - Fernando, Champika AU - Harding, John C.S. AU - Hill, Janet E. Y1 - 2016/10/19 PY - 2016 DA - 2017/01/01 N1 - doi: 10.1139/facets-2016-0018 DO - 10.1139/facets-2016-0018 T2 - FACETS JF - FACETS SP - 163 EP - 172 VL - 1 PB - Canadian Science Publishing N2 - A novel Brachyspira emerged in 2009 and has since become a production-limiting pathogen of pigs in North America. The name ?Brachyspira hampsonii? has been proposed for this novel taxon. ?Brachyspira hampsonii? is divided into two phylogenetically distinct clades based on the sequence of the NADH-oxidase (nox) gene, although the clinical disease associated with clades I and II is indistinguishable and phenotypic characteristics that discriminate the clades have not been determined. The objectives of the current study were to enhance the description of the provisional species ?B. hampsonii? with biochemical profiles and morphometric data from isolates affecting Canadian swine and to investigate potentially diagnostically informative characteristics for this emerging pathogen. Biochemical profiles of isolates from different commercial swine barns in Western Canada showed that biochemical profiles were insufficient to distinguish ?B. hampsonii? clades I and II from each other or from other pathogenic Brachyspira. Hippurate hydrolysis, previously reported as uniformly negative in ?B. hampsonii,? was variable among Canadian isolates. Spirochete dimensions and flagella numbers for ?B. hampsonii? overlapped with other Brachyspira species. Taken together, these results indicate that nox gene sequencing remains a preferred method for identification and discrimination of ?B. hampsonii? from other pig-associated Brachyspira spp. AB - A novel Brachyspira emerged in 2009 and has since become a production-limiting pathogen of pigs in North America. The name ?Brachyspira hampsonii? has been proposed for this novel taxon. ?Brachyspira hampsonii? is divided into two phylogenetically distinct clades based on the sequence of the NADH-oxidase (nox) gene, although the clinical disease associated with clades I and II is indistinguishable and phenotypic characteristics that discriminate the clades have not been determined. The objectives of the current study were to enhance the description of the provisional species ?B. hampsonii? with biochemical profiles and morphometric data from isolates affecting Canadian swine and to investigate potentially diagnostically informative characteristics for this emerging pathogen. Biochemical profiles of isolates from different commercial swine barns in Western Canada showed that biochemical profiles were insufficient to distinguish ?B. hampsonii? clades I and II from each other or from other pathogenic Brachyspira. Hippurate hydrolysis, previously reported as uniformly negative in ?B. hampsonii,? was variable among Canadian isolates. Spirochete dimensions and flagella numbers for ?B. hampsonii? overlapped with other Brachyspira species. Taken together, these results indicate that nox gene sequencing remains a preferred method for identification and discrimination of ?B. hampsonii? from other pig-associated Brachyspira spp. M3 - doi: 10.1139/facets-2016-0018 UR - https://doi.org/10.1139/facets-2016-0018 Y2 - 2024/03/28 ER - TY - JOUR T1 - Identification of Treponema hyodysenteriae and Treponema innocens using two four-hour identification systems AU - Achacha, M AU - Messier, S JO - Journal of Veterinary Diagnostic Investigation Y1 - 1991 VL - 3 SP - 211 ER - TY - JOUR T1 - 5500 Phages examined in the electron microscope AU - Ackermann, HW JO - Archives of Virology Y1 - 2007 VL - 152 SP - 227 ER - TY - JOUR T1 - Fluorescent in situ hybridization for detection of “Brachyspira hampsonii” in porcine colonic tissues AU - Burrough, ER AU - Wilberts, BL AU - Bower, LP AU - Jergens, AE AU - Schwartz, KJ JO - Journal of Veterinary Diagnostic Investigation Y1 - 2013 VL - 25 SP - 407 ER - TY - JOUR T1 - Bacteriophages induced from weakly beta-haemolytic human intestinal spirochaetes by mitomycin C AU - 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