TY  - JOUR
T1  - Identification of leaf-mining insects via DNA recovered from empty mines
AU  - Mlynarek, Julia J.
AU  - Kim, Jin-Hong
AU  - Heard, Stephen B.
Y1  - 2016/11/23
PY  - 2016
DA  - 2017/01/01
N1  - doi: 10.1139/facets-2016-0026
DO  - 10.1139/facets-2016-0026
T2  - FACETS
JF  - FACETS
SP  - 217
EP  - 224
VL  - 1
PB  - Canadian Science Publishing
N2  - We report the development of an improved method for the extraction and amplification of leaf miner DNA recovered from empty mines. Our method is simple, easy to use, and foregoes the time-consuming task of scraping out mines required by previous methods. We collected leaves with 1- and 2-day-old vacated mines, cut out and then ground the mined portions, and amplified the mtDNA COI barcode sequence using universal insect primers. We obtained high-quality sequences for 31% of our empty mines: 20% yielded sequences associated with a leaf miner species; and an additional 11% yielded sequences associated with whiteflies, mites, or fungi. Our improved method will facilitate ecological studies determining herbivore community dynamics and agricultural studies for pest monitoring and identification.
AB  - We report the development of an improved method for the extraction and amplification of leaf miner DNA recovered from empty mines. Our method is simple, easy to use, and foregoes the time-consuming task of scraping out mines required by previous methods. We collected leaves with 1- and 2-day-old vacated mines, cut out and then ground the mined portions, and amplified the mtDNA COI barcode sequence using universal insect primers. We obtained high-quality sequences for 31% of our empty mines: 20% yielded sequences associated with a leaf miner species; and an additional 11% yielded sequences associated with whiteflies, mites, or fungi. Our improved method will facilitate ecological studies determining herbivore community dynamics and agricultural studies for pest monitoring and identification.
M3  - doi: 10.1139/facets-2016-0026
UR  - https://doi.org/10.1139/facets-2016-0026
Y2  - 2024/04/26
ER  - 


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