TY  - JOUR
T1  - Substrate utilization by endophytic bacteria Paenibacillus polymyxa P2b-2R that may facilitate bacterial entrance and survival inside diverse plant hosts
AU  - Yang, Henry
AU  - Puri, Akshit
AU  - Padda, Kiran Preet
AU  - Chanway, Chris P.
Y1  - 2017/01/01
PY  - 2017
DA  - 2017/01/01
N1  - doi: 10.1139/facets-2016-0031
DO  - 10.1139/facets-2016-0031
T2  - FACETS
JF  - FACETS
SP  - 120
EP  - 130
VL  - 2
PB  - Canadian Science Publishing
N2  - Bacterial endophytes are thought to enter plants either through pre-existing openings in plant tissues or by creating openings by hydrolyzing major plant cell wall components. A lodgepole endophyte, Paenibacillus polymyxa P2b-2R, consistently formed endophytic colonies when inoculated in diverse plant hosts, viz., lodgepole pine, western red cedar, corn, canola, and tomato. We were interested to know, whether or not this bacterial strain possesses enzymes that can hydrolyze three major plant cell wall components namely cellulose, xylan, and pectin to facilitate entrance into the host plants. Using a BIOLOG assay, we also tested this bacterial strain?s ability to utilize carbon sources that might facilitate its entrance and hence its survival inside host plants. Paenibacillus polymyxa P2b-2R hydrolyzed sodium carboxymethylcellulose, beechwood xylan, and sodium polypectate and utilized 39 of the 95 carbon sources (41%) tested. Of the 39 carbon substrates oxidized by P2b-2R, the ?carbohydrates? group represents the largest source of utilizable carbon (23 out of 39). Thus, it can be concluded that P. polymyxa P2b-2R is able to degrade major cell wall components (cellulose, xylan, and pectin) and utilize some of the available carbon substrates, possibly to gain entry and survive inside the plant and form endophytic colonies thereafter.
AB  - Bacterial endophytes are thought to enter plants either through pre-existing openings in plant tissues or by creating openings by hydrolyzing major plant cell wall components. A lodgepole endophyte, Paenibacillus polymyxa P2b-2R, consistently formed endophytic colonies when inoculated in diverse plant hosts, viz., lodgepole pine, western red cedar, corn, canola, and tomato. We were interested to know, whether or not this bacterial strain possesses enzymes that can hydrolyze three major plant cell wall components namely cellulose, xylan, and pectin to facilitate entrance into the host plants. Using a BIOLOG assay, we also tested this bacterial strain?s ability to utilize carbon sources that might facilitate its entrance and hence its survival inside host plants. Paenibacillus polymyxa P2b-2R hydrolyzed sodium carboxymethylcellulose, beechwood xylan, and sodium polypectate and utilized 39 of the 95 carbon sources (41%) tested. Of the 39 carbon substrates oxidized by P2b-2R, the ?carbohydrates? group represents the largest source of utilizable carbon (23 out of 39). Thus, it can be concluded that P. polymyxa P2b-2R is able to degrade major cell wall components (cellulose, xylan, and pectin) and utilize some of the available carbon substrates, possibly to gain entry and survive inside the plant and form endophytic colonies thereafter.
M3  - doi: 10.1139/facets-2016-0031
UR  - https://doi.org/10.1139/facets-2016-0031
Y2  - 2024/04/20
ER  - 


TY  - JOUR
T1  - Plant cell-wall degrading hydrolytic enzymes of Gluconacetobacter diazotrophicus
AU  - Adriano-Anaya, M
AU  - Salvador-Figueroa, M
AU  - Ocampo, JA
AU  - Garcia-Romera, I
JO  - Symbiosis
Y1  - 2005
VL  - 40
IS  - 3
SP  - 151
ER  - 

TY  - JOUR
T1  - Detection of GFP-labelled Paenibacillus polymyxa in autofluorescing pine seedling tissues
AU  - Anand, R
AU  - Chanway, C
JO  - Biology and Fertility of Soils
Y1  - 2013
VL  - 49
IS  - 1
SP  - 111
ER  - 

TY  - JOUR
T1  - N2-fixation and growth promotion in cedar colonized by an endophytic strain of Paenibacillus polymyxa
AU  - Anand, R
AU  - Chanway, C
JO  - Biology and Fertility of Soils
Y1  - 2013
VL  - 49
IS  - 2
SP  - 235
ER  - 

TY  - JOUR
T1  - N2-fixation and seedling growth promotion of lodgepole pine by endophytic Paenibacillus polymyxa
AU  - Anand, R
AU  - Grayston, S
AU  - Chanway, C
JO  - Microbial Ecology
Y1  - 2013
VL  - 66
IS  - 2
SP  - 369
ER  - 

TY  - JOUR
T1  - Endophytic microorganisms: a review on insect control and recent advances on tropical plants
AU  - Azevedo, JL
AU  - Maccheroni, W
AU  - Pereira, JO
AU  - Araújo, WL
JO  - Electronic Journal of Biotechnology
Y1  - 2000
VL  - 3
IS  - 1
SP  - 40
ER  - 

TY  - JOUR
T1  - Oligogalacturonide signal transduction, induction of defense-related responses and protection of grapevine against Botrytis cinerea
AU  - Aziz, A
AU  - Heyraud, A
AU  - Lambert, B
JO  - Planta
Y1  - 2004
VL  - 218
IS  - 5
SP  - 767
ER  - 

TY  - JOUR
T1  - Isolation and identification of diazotrophic bacteria from internal tissues of Pinus contorta and Thuja plicata
AU  - Bal, A
AU  - Anand, R
AU  - Berge, O
AU  - Chanway, CP
JO  - Canadian Journal of Forest Research
Y1  - 2012
VL  - 42
IS  - 4
SP  - 807
ER  - 

TY  - JOUR
T1  - Sleuthing out bacterial identities
AU  - Bochner, BR
JO  - Nature
Y1  - 1989
VL  - 339
IS  - 6220
SP  - 157
ER  - 

TY  - BOOK
AU  - Boddey, RM
AU  - Urquiaga, S
AU  - Reis, V
AU  - Döbereiner, J
AU  - Polsinelli, M
AU  - Materassi, R
AU  - Vincenzini, M
TI  - Nitrogen fixation
Y1  - 1991
SP  - 105
ER  - 

TY  - JOUR
T1  - Structure and functions of the bacterial microbiota of plants
AU  - Bulgarelli, D
AU  - Schlaeppi, K
AU  - Spaepen, S
AU  - van Themaat, EVL
AU  - Schulze-Lefert, P
JO  - Annual Review of Plant Biology
Y1  - 2013
VL  - 64
SP  - 807
ER  - 

TY  - JOUR
T1  - Endophytic bacterial communities in ginseng and their antifungal activity against pathogens
AU  - Cho, KM
AU  - Hong, SY
AU  - Lee, SM
AU  - Kim, YH
AU  - Kahng, GG
AU  - Lim, YP
JO  - Microbial Ecology
Y1  - 2007
VL  - 54
IS  - 2
SP  - 341
ER  - 

TY  - JOUR
T1  - Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN
AU  - Compant, S
AU  - Reiter, B
AU  - Sessitsch, A
AU  - Nowak, J
AU  - Clément, C
AU  - Ait Barka, E
JO  - Applied and Environmental Microbiology
Y1  - 2005
VL  - 71
IS  - 4
SP  - 1685
ER  - 

TY  - BOOK
AU  - Döbereiner, J
AU  - Reis, V
AU  - Lazarine, AC
AU  - Bothe, H
AU  - de Bruijn, FJ
AU  - Newton, WE
TI  - Nitrogen fixation: hundred years after
Y1  - 1988
SP  - 717
ER  - 

TY  - JOUR
T1  - A nitrogen-fixing endophyte of sugarcane stems (a new role for the apoplast)
AU  - Dong, Z
AU  - Canny, MJ
AU  - McCully, ME
AU  - Roboredo, MR
AU  - Cabadilla, CF
AU  - Ortega, E
JO  - Plant Physiology
Y1  - 1994
VL  - 105
IS  - 4
SP  - 1139
ER  - 

TY  - JOUR
T1  - Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice
AU  - Fouts, DE
AU  - Tyler, HL
AU  - de Boy, RT
AU  - Daugherty, S
AU  - Ren, Q
AU  - Badger, JH
JO  - PLoS Genetics
Y1  - 2008
VL  - 4
IS  - 7
SP  - e1000141
ER  - 

TY  - JOUR
T1  - Host-derived signals activate plant innate immunity
AU  - Galletti, R
AU  - de Lorenzo, G
AU  - Ferrari, S
JO  - Plant Signaling and Behavior
Y1  - 2009
VL  - 4
IS  - 1
SP  - 33
ER  - 

TY  - JOUR
T1  - Distinct microbial communities within the endosphere and rhizosphere of Populus deltoides roots across contrasting soil types
AU  - Gottel, NR
AU  - Castro, HF
AU  - Kerley, M
AU  - Yang, Z
AU  - Pelletier, DA
AU  - Podar, M
JO  - Applied and Environmental Microbiology
Y1  - 2011
VL  - 77
IS  - 17
SP  - 5934
ER  - 

TY  - JOUR
T1  - Bacterial endophytes in agricultural crops
AU  - Hallmann, J
AU  - Quadt-Hallmann, A
AU  - Mahaffee, WF
AU  - Kloepper, JW
JO  - Canadian Journal of Microbiology
Y1  - 1997
VL  - 43
IS  - 10
SP  - 895
ER  - 

TY  - JOUR
T1  - Root colonization and systemic spreading of Azoarcus sp. strain BH72 in grasses
AU  - Hurek, T
AU  - Reinhold-Hurek, B
AU  - van Montagu, M
AU  - Kellenberg, E
JO  - Journal of Bacteriology
Y1  - 1994
VL  - 176
IS  - 7
SP  - 1913
ER  - 

TY  - JOUR
T1  - Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus
AU  - James, EK
AU  - Reis, VM
AU  - Olivares, FL
AU  - Baldani, JI
AU  - Döbereiner, J
JO  - Journal of Experimental Botany
Y1  - 1994
VL  - 45
IS  - 6
SP  - 757
ER  - 

TY  - JOUR
T1  - Rhizosphere and endophytic bacteria for induction of systemic resistance of banana plantlets against bunchy top virus
AU  - Kavino, M
AU  - Harish, S
AU  - Kumar, N
AU  - Saravanakumar, D
AU  - Damodaran, T
AU  - Sooianathasundaram, K
JO  - Soil Biology and Biochemistry
Y1  - 2007
VL  - 39
IS  - 5
SP  - 1087
ER  - 

TY  - BOOK
AU  - Lehninger, AL
TI  - Biochemistry: the molecular basis of cell structure and function
Y1  - 1975
ER  - 

TY  - JOUR
T1  - Cell-associated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii
AU  - Mateos, PF
AU  - Jimenez-Zurdo, JI
AU  - Chen, J
AU  - Squartini, AS
AU  - Haack, SK
AU  - Martinez-Molina, E
JO  - Applied and Environmental Microbiology
Y1  - 1992
VL  - 58
IS  - 6
SP  - 1816
ER  - 

TY  - JOUR
T1  - Population dynamics of Gluconacetobacter diazotrophicus in sugarcane cultivars and its effect on plant growth
AU  - Muñoz-Rojas, J
AU  - Caballero-Mellado, J
JO  - Microbial Ecology
Y1  - 2003
VL  - 46
IS  - 4
SP  - 454
ER  - 

TY  - JOUR
T1  - Host-pathogen interactions: XXII. A galacturonic acid oligosaccharide from plant cell walls elicits phytoalexins
AU  - Nothnagel, EA
AU  - McNeil, M
AU  - Albersheim, P
AU  - Dell, A
JO  - Plant Physiology
Y1  - 1983
VL  - 71
IS  - 4
SP  - 916
ER  - 

TY  - JOUR
T1  - Effect of GFP tagging of Paenibacillus polymyxa P2b-2R on its ability to promote growth of canola and tomato seedlings
AU  - Padda, KP
AU  - Puri, A
AU  - Chanway, CP
JO  - Biology and Fertility of Soils
Y1  - 2016
VL  - 52
IS  - 3
SP  - 377
ER  - 

TY  - JOUR
T1  - Plant growth promotion and nitrogen fixation in canola (Brassica napus) by an endophytic strain of Paenibacillus polymyxa and its GFP-tagged derivative in a long-term study
AU  - Padda, KP
AU  - Puri, A
AU  - Chanway, CP
JO  - Botany
Y1  - 2016
VL  - 94
IS  - 12
SP  - 1209
ER  - 

TY  - JOUR
T1  - Can a diazotrophic endophyte originally isolated from lodgepole pine colonize an agricultural crop (corn) and promote its growth?
AU  - Puri, A
AU  - Padda, KP
AU  - Chanway, CP
JO  - Soil Biology and Biochemistry
Y1  - 2015
VL  - 89
SP  - 210
ER  - 

TY  - JOUR
T1  - Evidence of nitrogen fixation and growth promotion in canola (Brassica napus L.) by an endophytic diazotroph Paenibacillus polymyxa P2b-2R
AU  - Puri, A
AU  - Padda, KP
AU  - Chanway, CP
JO  - Biology and Fertility of Soils
Y1  - 2016
VL  - 52
IS  - 1
SP  - 119
ER  - 

TY  - JOUR
T1  - Seedling growth promotion and nitrogen fixation by a bacterial endophyte Paenibacillus polymyxa P2b-2R and its GFP derivative in corn in a long-term trial
AU  - Puri, A
AU  - Padda, KP
AU  - Chanway, CP
JO  - Symbiosis
Y1  - 2016
VL  - 69
IS  - 2
SP  - 123
ER  - 

TY  - JOUR
T1  - Screening and evaluation of probiotics as a biocontrol agent against pathogenic Vibrios in marine aquaculture
AU  - Ravi, AV
AU  - Musthafa, KS
AU  - Jegathammbal, G
AU  - Kathiresan, K
AU  - Pandian, SK
JO  - Letters in Applied Microbiology
Y1  - 2007
VL  - 45
IS  - 2
SP  - 219
ER  - 

TY  - JOUR
T1  - An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp. strain BH72
AU  - Reinhold-Hurek, B
AU  - Maes, T
AU  - Gemmer, S
AU  - van Montagu, M
AU  - Hurek, T
JO  - Molecular Plant–Microbe Interactions
Y1  - 2006
VL  - 19
IS  - 2
SP  - 181
ER  - 

TY  - JOUR
T1  - A single medium for the isolation of acetylene-reducing (dinitrogen-fixing) bacteria from soils
AU  - Rennie, RJ
JO  - Canadian Journal of Microbiology
Y1  - 1981
VL  - 27
IS  - 1
SP  - 8
ER  - 

TY  - JOUR
T1  - Endophytic colonization of spruce by plant growth-promoting rhizobacteria
AU  - Shishido, M
AU  - Breuil, C
AU  - Chanway, CP
JO  - FEMS Microbiology Ecology
Y1  - 1999
VL  - 29
SP  - 191
ER  - 

TY  - JOUR
T1  - External and internal root colonization of lodgepole pine seedlings by two growth-promoting Bacillus strains originated from different root microsites
AU  - Shishido, M
AU  - Loeb, BM
AU  - Chanway, CP
JO  - Canadian Journal of Microbiology
Y1  - 1995
VL  - 41
IS  - 8
SP  - 707
ER  - 

TY  - JOUR
T1  - Paenibacillus polymyxa invades plant roots and forms biofilms
AU  - Timmusk, S
AU  - Grantcharova, N
AU  - Wagner, EGH
JO  - Applied and Environmental Microbiology
Y1  - 2005
VL  - 71
IS  - 11
SP  - 7292
ER  - 

TY  - JOUR
T1  - Effects of Paenibacillus polymyxa inoculation and different soil nitrogen treatments on lodgepole pine seedling growth
AU  - Yang, H
AU  - Puri, A
AU  - Padda, KP
AU  - Chanway, CP
JO  - Canadian Journal of Forest Research
Y1  - 2016
VL  - 46
IS  - 6
SP  - 816
ER  -