Microplastic abundance and distribution in the open water and sediment of the Ottawa River, Canada, and its tributaries
Abstract
Microplastic pollution is prevalent in the Ottawa River, with all open water samples (n = 62) and sediment samples (n = 10) containing microplastics. The median microplastic concentration of nearshore 100 L water samples was 0.1 fragments per L (ranged between 0.05 and 0.24 fragments per L). The larger volume Manta trawls samples taken in the middle of the Ottawa River had an overall mean concentration of plastics of 1.35 fragments per m3. Plastic concentrations were significantly higher downstream of the wastewater treatment plant (1.99 fragments per m3) compared with upstream of the effluent output (0.71 fragments per m3), suggesting that the effluent plume is a pathway for plastic pollution to the Ottawa River. The mean concentration of microplastic fragments recovered in the sediment samples was 0.22 fragments per g dry weight. The abundance of microplastics in the sediment was not significantly related to the mean particle size or the organic content of the sediment. The most common form of plastic particles found was microfibers. These made up between 70% and 100% of all plastic particles observed, although plastic microbeads and secondary plastic fragments were also recovered.
Introduction
Plastics are ubiquitous in today’s society, and plastic pollution is one of the defining legacies of humans on the earth. In fact, over the last half century, plastic pollution has been synonymous with human society to the point that it has recently been proposed as a defining feature of the Anthropocene, a newly proposed geological epoch in which humans are a dominant force affecting the earth system (Waters et al. 2016).
In 2015, global plastic production was estimated at 322 million metric tons (Plastics—the Facts 2016), and the vast majority of plastic products are inexpensive and intended to be disposable. This has resulted in massive quantities of plastics being produced and thus disposed of on an annual basis. Plastic polymers do not biodegrade at timescales relevant for human society (i.e., multiple centuries to millennia or longer), but are broken down by UV radiation and mechanical forces into smaller and smaller strands of polymers (Barnes et al. 2009). This breakdown, but lack of biological degradation, results in a buildup of small plastic fragments, which has been most extensively studied in marine environments (Cole et al. 2011). These small plastic particles can have negative impacts on aquatic ecosystems including concentrating other contaminants in the system (Gregory 1996; Rios et al. 2007) and being ingested by animals, including apex predators, reducing their fitness and increasing mortality (Provencher et al. 2015; Sigler 2014; Lönnstedt and Eklöv 2016; Holland et al. 2016).
In aquatic pollution studies, plastic fragments are divided into two main groups based on size: microplastics (≤5 mm) and macroplastics (>5 mm; Masura et al. 2015). Microplastics found in natural systems can come from a variety of sources. Primary microplastics are manufactured by industry; for example, microbeads in cosmetics, toothpaste, and face wash, or microfibers from synthetic clothing and rope (Thompson et al. 2009). Secondary microplastics are the result of the breakdown of larger plastic fragments by erosion, solar radiation, water currents, and freezing and thawing (Barnes et al. 2009).
Microplastic pollution in marine environments is well established (Cole et al. 2011). It is only recently, however, that the presence of microplastics in freshwater systems has been reported (Eriksen et al. 2013; Castañeda et al. 2014; Baldwin et al. 2016), and there is still a limited understanding of where microplastics are occurring in freshwater systems and the controls on their abundance and distribution (Wagner et al. 2014; Anderson et al. 2016). In Canada, few studies have examined plastic pollution of freshwater outside of the Great Lakes/St. Lawrence system, and there is a lack of knowledge of the presence and concentration of plastics in Canadian freshwater systems (Anderson et al. 2016). The goals of this study were threefold. First we quantified the abundance of microplastics in the open water of the Ottawa River and its larger tributaries within the boundaries of the National Capital Region (including the cities of Ottawa, Ontario, and Gatineau, Quebec) to establish microplastic concentrations for this system. We then investigated microplastic pollution upstream and downstream of the city of Ottawa effluent plume in the Ottawa River to test if this effluent plume is a source of microplastics to the Ottawa River. Finally, we quantified the abundance of microplastics in river sediment samples along a depositional gradient to test if microplastic concentrations in the sediments were related to the depositional environment of the river.
Study region
The Ottawa River is a recently designated Canadian Heritage River and holds substantial cultural, ecological, and economic importance to Canada. The river flows for approximately 1271 km, west from northern Quebec into Lake Temiskaming, and then southeast along the border of Ontario and Quebec before emptying into Lake of Two Mountains and the St. Lawrence River in southeastern Quebec. The catchment area of the Ottawa River is 146 300 km2, larger than the size of England, and encompasses large areas of wetlands, boreal forest, and temperate forest in its southern reaches. Sampling took place in Canada’s National Capital Region, which includes the Canadian capital city of Ottawa as well as the neighboring city of Gatineau (Quebec) and smaller towns and rural communities with an approximate population of 1.23 million people.
Methods
Field sampling
To quantify the abundance of microplastics, surface water samples were collected in the summer of 2016 from sampling sites chosen to give a good representation of the Ottawa River watershed within the National Capital Region, both below and above the city of Ottawa’s wastewater treatment plant. These sites included locations along the Ottawa River, Rideau River, Rideau Canal and Brewery Creek, and effluent ponds at the city of Ottawa wastewater treatment plant. Water sampling for plastics was performed using two methods: (1) a nearshore bottle sampling method that filtered 100 L by hand, making it highly portable; and (2) a Manta trawl method that sampled a greater volume of water (∼100 000 L) and was ideal for comparisons with other microplastic research in large lake and marine environments, but challenging to deploy in a variety of shallower sites. The bottle sampling method was performed nearshore in flowing water at a depth of 0.5 m below the water’s surface. At each bottle sampling site, 4 L plastic jugs were used to filter 100 L of water through 100 μm nylon mesh. This was repeated three times at each location. Before use in the field, each filter was examined under a stereomicroscope to ensure that there was no microplastic contamination on the filter and then placed into a sterile Whirl-Pak bag until use. In the field, the filters were carefully removed from the Whirl-Pak bag and placed on the end of a metal cylinder and secured at the base by a hose clamp before water filtration. After each 100 L sample of water was filtered, the filters were carefully removed from the metal cylinder and placed directly back into a Whirl-Pak bag and sealed. Samples were kept refrigerated at 4 °C until processed in the laboratory.
Three 100 L effluent samples were also collected from the city of Ottawa wastewater treatment plant for microplastic analysis. Effluent samples were obtained from the final holding tank at the wastewater treatment plant before the effluent is released into the river. Effluent samples were collected from approximately 15 cm below the surface using an ISCO peristaltic pump and filtered using a 100 μm nylon mesh similar to the bottle sampling method used in the river water sampling.
To sample a larger volume of water specifically upstream and downstream of the effluent plume from the city of Ottawa’s wastewater treatment plant, Manta trawls, using a 100 μm mesh size, were taken from a boat above and below the Ottawa wastewater treatment effluent plume. The Manta net was deployed three times both above and below the effluent plume off the stern of the boat at surface depth for 20 min time intervals. To estimate the volume of water entering the Manta net, the flow of water entering the net was measured in meters per second using a Global Water flow meter. The total volume of water sampled by the Manta net varied between 84 and 181 m3 (mean = 128 m3, standard deviation = 37 m3) and was estimated based on the size of the Manta net opening (area = 0.116 m2), the average flow rate of the sampling interval, and the duration of the sampling interval. Following each Manta trawl, the material retained in the Manta net was washed through a clean 30 μm nylon mesh filter that was then sealed in a Whirl-Pak bag and kept refrigerated until processing. Between each sample, the Manta net was backwashed with river water, and the sock at the end of the Manta net was washed with deionized water in an effort to limit cross-contamination between sampling locations.
To quantify microplastic concentrations in the sediment along a depositional gradient, 10 sediment samples were collected in the fall of 2015 near Petrie Island in the Ottawa River using an Ekman bottom grab sampler with a sample volume of 3.5 L. The sampling site was selected because of its location downstream of the city of Ottawa’s wastewater treatment plant and because there is a large depositional gradient from sandy bottom sediment to fine silts and clays to test if plastic concentrations varied with depositional environment. Between each sample, the Ekman sampler was rinsed with river water and the river sediment was placed in sealed plastic bags and refrigerated until processing in the laboratory.
Laboratory and statistical analyses
In the laboratory, each surface water sample and effluent sample was washed from the mesh filter into a beaker using distilled water and underwent a wet hydrogen peroxide (H2O2) oxidation using a 30% H2O2 solution heated to 80 °C for 7 h to remove organic material from the sample. Following the wet oxidation, the resulting sample was filtered a second time through a clean 100 μm filter. The content in the filter was then backwashed with distilled water into a Petri dish and examined under a Leica stereomicroscope at 40× magnification for microplastics. The microplastics observed were enumerated, and larger fragments were isolated from the sample with forceps for permanent storage. It should be noted that this filtering and visual inspection methodology does not take into account microplastic particles less than 100 μm that would pass through the filter and would have been difficult to reliably identify under a stereomicroscope at 40× magnification.
In addition to freshwater and effluent samples collected in the field, samples of tap water taken from Carleton University were processed in the same manner as the river water samples to serve as control samples for possible contamination with microplastics in the laboratory or during processing. For these control samples, 100 L of tap water were filtered through the same apparatus using the same 4 L Nalgene plastic bottles as the river water samples. These control samples then underwent wet H2O2 oxidation at the same time and location as the field samples using the same equipment. The control samples were then inspected under the stereomicroscope and any microplastic particles observed were counted.
The sediment samples required greater processing than the surface water samples to isolate microplastics because the sediment samples contained much more material retained by the initial sieving. Sediment sample preparation for the isolation of microplastics followed Masura et al. (2015) but omitted the first density separation. A 400 g sample of wet sediment was dried in an oven at 100 °C for 24 h to calculate the dry mass of the sediment. The dried sediment sample was then dispersed using a 5% sodium hexametaphosphate solution and mixed with a magnetic stir bar for 1 h. The dispersed sample was then passed through a 0.3 mm sieve and washed clean. The material retained in the 0.3 mm sieve was transferred to a beaker and underwent wet H2O2 oxidation using 300 mL of 30% H2O2 to remove organic material. The sample material was then sieved through a 100 μm sieve and washed, and the material remaining in the sieve was then transferred to a 500 mL beaker. The sample material then underwent a density separation using a sodium chloride (NaCl) solution of 6 g of salt per 20 mL of sample solution to separate plastic material from the denser sediment (Masura et al. 2015). The salt solution and material were then transferred to a separation funnel and allowed to settle for 30 min. The sediment that settled at the bottom of the separation funnel was then removed, examined for microplastics under a stereomicroscope, and discarded. The solution that remained in the separation funnel was then passed through a 100 μm sieve, thoroughly washed with deionized water to remove all salt, and then backwashed into a Petri dish and examined for microplastics under a Leica stereomicroscope at 40× magnification. Observed microplastic particles were counted, and larger fragments were removed from the Petri dish with forceps and permanently stored.
The organic content and grain size of the sediment were calculated to characterize the depositional environment of the sample site. To determine the organic content of the sediment, a loss-on-ignition analysis was performed using standard methodology (Heiri et al. 2001). Dry sediment (1 cc) was heated in a muffle furnace to 550 °C for 4 h, and the organic content of the sediment was estimated based on the weight loss of the sediment. The mean particle size of the sediment sample was determined using a Coulter laser diffraction analysis conducted on prepared sediment. For particle size analysis, 1 cc of sediment underwent wet H2O2 oxidation at 80 °C for 7 h to remove organic matter. Following oxidation, a 5% sodium hexametaphosphate solution was added to the sample as a dispersant for 24 h before the sample underwent particle size analysis. The arithmetic mean particle size of the sample was calculated using GRADISTAT version 8 (Blott and Pye 2001).
For the Manta net sample, to test if microplastic concentrations were greater downstream from the city of Ottawa effluent plume, a two-sided t test was carried out comparing samples above and below the effluent plume. For the sediment samples, to test if the depositional environment of the system is correlated to microplastic abundance, a nonparametric Spearman’s rank correlation test was performed to compare the abundance of microplastics (number of pieces of microplastic per kg of dry sediment) to the organic matter content and mean particle size of the sediment sample. All statistical analyses and graphing were carried out in R version 3.3.1 (R Development Core Team 2016), and the map was produced using ARCMap version 10.4.1 by Esri.
Results
Microplastics were found in every single surface water sample collected from the Ottawa River and its tributaries (n = 62). With the bottle sampling method, plastic concentrations ranged from a low of 0.05 plastic fragments per L at the confluence of the Madawaska River into the Ottawa River to a high of 0.24 fragments per L in the Rideau River (site a in Fig. 1). The greatest median concentration of plastics was observed in water samples from the Rideau Canal (0.19 fragments per L), followed closely by samples collected at Petrie Island in the Ottawa River (0.18 fragments per L), which is downstream from where the Ottawa wastewater treatment plant releases effluent into the Ottawa River (site h in Fig. 1). The overall median plastic concentration observed for surface water samples using the 100 L filtering technique was 0.1 fragments per L. Interestingly, samples collected directly from the effluent tank using a pump had some of the lowest plastic concentrations observed in all of our samples (median = 0.07, n = 3), suggesting that at the time, plastic concentrations were low at our sampling depth.
Fig. 1.
Using the bottle sampling method, not only were plastic fragments detected in all of our surface water samples, but they were also detected in approximately 80% of our tap water samples (9 out of 11 samples). Although the median concentration of plastics in the tap water samples (0.02 fragments per L) is an order of magnitude lower than the median plastic concentration of the field samples (0.1 fragments per L) and there was significantly more plastic in the field samples compared with the tap water controls based on a Kruskal–Wallis test (p < 0.001), these results highlight the potential for the contamination of microplastic samples and the need for laboratory controls to assess the level of contamination.
The vast majority of plastic fragments recovered were microfibers (Fig. 2). Using the bottle sampling method, >95% of plastic fragments recovered were microfibers with the remainder of the sample consisting of microbeads and unidentified plastic fragments. The Manta net sampling was also dominated by plastic microfibers (mean 73% of plastic particles), but there was a greater proportion of microbeads (mean 7% of plastic particles) and other plastic fragments (mean 20% of plastic fragments).
Fig. 2.
The Manta trawl samples allowed for a much larger volume of water to be filtered than the bottle sampling method, giving a better approximation of plastic concentrations in the open water environment but at a reduced spatial coverage because it requires that the site is accessible by boat. Each 20 min Manta trawl filtered between approximately 84 000 and 180 000 L of water depending on the velocity of the river’s flow and the speed of the boat. Based on the Manta trawls, plastic concentrations in the Ottawa River below the effluent output (1.99 fragments per m3) were 2.8 times greater than plastic concentrations above the effluent output (0.71 fragments per m3) and statistically significant based on a two-sided t test (t = −4.97, df = 3.99, p = 0.008). Based on the Manta trawl sampling, the overall mean concentration of plastics in the open water of the Ottawa River was 1.35 fragments per m3 (Fig. 3). Estimates of plastic concentrations based on the Manta trawl are systematically lower than those estimates based on the bottle sampling method, which may reflect differences in sampling location (e.g., middle of river vs. edge of river) or in sampling methodology (small vs. large volume samples).
Fig. 3.
Microplastics were recovered from every sediment sampling location (Fig. 4). Similar to the open water samples, the microplastics observed in the sediment samples were dominated by microfibers (>95% of fragments), but some microbeads and other plastic fragments were also observed (Fig. 2). The mean concentration of microplastic fragments recovered in all 10 sediment samples was 33 fragments per 400 g wet weight of sediment (or 0.22 fragments per g dry weight). The maximum microplastic abundance observed was 56 fragments per 400 g wet weight (or 0.45 fragments per g dry weight). Although the samples with the greatest microplastic concentrations were those with lower mean particle size and higher organic content, there was no significant correlation between the number of pieces of microplastic per g dry weight or mean grain size (p = 0.51, ρ = −0.24; Fig. 4). The percent organic matter content was also not significantly related to the number of microplastic fragments per g dry weight (p = 0.84, ρ = −0.08; Fig. 4).
Fig. 4.
Discussion
Our research shows that plastic pollution is prevalent in the Ottawa River and its tributaries, with microplastics recovered in every water and sediment sample analyzed. Based on Manta net sampling, plastic concentration in the Ottawa River was greater than that reported in the Great Lakes (Eriksen et al. 2013), but less than the tributaries to the Great Lakes or more heavily populated rivers in the United States (Moore et al. 2002; Baldwin et al. 2016) (Fig. 3). Although microplastics were present in every sample, sampling methodology influenced the estimated microplastic concentration of the river, with the smaller volume bottle samples (100 L) taken nearshore being an order of magnitude greater than the open water samples taken with a Manta trawl. Therefore, to compare microplastic pollution among studies it is important that similar methodologies are used. For microplastic concentrations in the sediment, the depositional environment (mean particle size) and organic content of the sediment had little influence on the concentration of plastic in the sediment, suggesting that other factors are important for predicting microplastic deposition in the sediment.
Concentrations of microplastics reported in this study (mean of 1.35 fragments per m3 based on the larger volume Manta net sampling) are greater than have been reported in the Great Lakes (Eriksen et al. 2013), the San Gabriel River in the United States (0.002 fragments per m3; Moore et al. 2002), and the Danube River (0.8 fragments per m3; Lechner et al. 2014), but generally lower than reported in the North Shore Channel in Chicago, Illinois, USA (1.94–17.93 fragments per m3; McCormick et al. 2014), and much lower than has been reported from heavily urbanized California rivers (30–12 000 fragments per m3; Moore et al. 2002). Similar to marine environments, as predicted by Jambeck et al. (2015), given the increasing production and use of plastic, it is likely that plastic pollution of freshwater systems including the Ottawa River will increase in the future.
The types of plastic fragments, dominated by red and blue microfibers, found in the sediment samples resembled what was recovered in the open water sampling, suggesting that the microplastics in the sediment settled out of the water column despite the plastics being less dense than freshwater. It may be that microplastics in the open water environment aggregate together with particulate organic and settle out of the water column. Comparing the abundance of microplastics recovered in the Ottawa River sediment with other freshwater studies is somewhat complicated by the unit of measure. We found a mean of 0.22 plastic fragments per g dry weight in the Ottawa River, which is comparable with what has been reported in German rivers (0.34–0.64 fragments per g dry weight; Wagner et al. 2014). Other freshwater studies of microplastics in sediment have tended to express their values on an areal basis (Anderson et al. 2016), but as an approximate comparison, our values appear greater than those from Great Lake sediments 0–34 fragments per m2 (Zbyszewski et al. 2014) but lower than sediments from the St. Lawrence River (mean of 13 822 fragments per m2; Castañeda et al. 2014).
The concentration of microplastics recovered in the sediment samples was not significantly related to either the sediment particle size or the organic content of the sediment. It therefore appears that the depositional environment has little influence on the concentration of microplastics settling out of the water column into the sediment. It may be that the majority of these plastic particles are settling out of the water in larger aggregates of microplastics mixed with particulate organic and inorganic material, giving the aggregate a greater density and allowing the plastic to settle out under greater water velocities, although this would need to be examined in another study. An additional possibility is that our sample size was too small to give us the statistical power to detect a significant trend given the variability in the data. A post hoc power analysis indicated that a sample size of 60 would be required to adequately test the effect of depositional environment on microplastic concentrations given the variability observed in this study.
Although the impact of microplastics on freshwater ecosystems is poorly understood (Wagner et al. 2014; Anderson et al. 2016), it is clear that some freshwater organisms are consuming this plastic pollution (Holland et al. 2016), and given what has been observed in marine environments (Provencher et al. 2015; Lönnstedt and Eklöv 2016) it is plausible that they are having some detrimental impacts on freshwater organisms. Few toxicological studies have investigated the impact of microplastics on freshwater organisms and this remains a major knowledge gap in our assessment of water quality for the protection of aquatic life (Anderson et al. 2016). Au et al. (2015) investigated the impact of microplastics on Hyalella azteca, an amphipod crustacean, and found that microfibers were significantly more toxic than microbeads to the organism due to the longer residence time of the fibers in the gut of the organism, although all forms of microplastic displayed a toxic effect. This is worrying given that the vast majority of plastic particles recovered in our study were microfibers, but at concentrations that were orders of magnitude lower than those used by Au et al. (2015) in their acute and chronic exposure experiments. The greater prevalence and toxicity of plastic microfibers is also a concern because this form of plastic pollution is even more difficult to control than secondary plastics and microbeads, whose concentrations may be partially reduced through improved waste management and public policy phasing out the use of microbeads in cosmetics. Microfibers on the other hand come from multiple sources (Browne et al. 2011) and are easily transported into aquatic system both by runoff and by atmospheric deposition (Browne et al. 2011; Gasperi et al. 2015).
It is also of note that many of our tap water control samples also had a small amount of microplastic in the sample (one or two fibers per 100 L filtered). It is plausible that these fibers were in the tap water and are not a source of contamination, as microplastic pollution has even been reported in beer purchased in a German supermarket (Liebezeit and Liebezeit 2014). The city of Ottawa gets its drinking water from the Ottawa River; however, the city filters their drinking water through a 1.0 μm filter, which should remove all but the smallest plastic fragments from the river water. Although post-filter contamination of the drinking water with microplastics is entirely plausible, another possibility is that these samples became contaminated with small amounts of microplastics during the sampling or in the lab. Although efforts were taken to prevent contamination during the sampling and in the lab (using pre-inspected filters, sterile bags, and always keeping samples covered), microplastic fibers are common in the air and atmosphere, with indoor air samples reporting microplastic fiber concentrations in the range of 3–15 particles per m3 (Gasperi et al. 2015). It is an important point that the microplastics reported in air quality testing were always microfibers (Gasperi et al. 2015), which were the only types of plastic particles observed in our tap water samples. Due to the high prevalence of microplastic fibers in the air, it is important when sampling aquatic microplastics to limit open contact with the air as much as possible to limit contamination. It is also important to consider that smaller sample sizes are more prone to having large relative errors due to lab contamination by a single microplastic fiber. Therefore, whenever possible, large volume samples should be employed to get the best estimate of microplastic concentrations in the system. To account for possible contamination, we recommend running control samples such as the tap water samples used in this study. This should provide a representative background contamination level based on plastic concentrations at the sampling site and in the laboratory’s air.
Conclusions
Our study has shown that plastic pollution of the Ottawa River and its major tributaries in Canada’s National Capital Region is prevalent and at concentrations greater than have been reported for many other freshwater systems including the Great Lakes. Our study contributes to a growing body of research (reviewed by Wagner et al. 2014 and Anderson et al. 2016) that suggests that microplastic pollution of freshwater ecosystems may be ubiquitous. It is therefore important that we develop reliable and repeatable methods for sampling and the reporting of microplastic pollution, and that we increase our understanding of the spatial and temporal variability of plastic pollution in freshwater systems. In addition, the effects of microplastic pollution, if any, on freshwater ecosystems and human health remain an important knowledge gap in our management of water quality for the protection of aquatic life.
Acknowledgements
We would like to thank Brian Bezaire for making the wastewater effluent sampling possible. This research was supported by an NSERC Discovery Grant to JCV, an NSERC USRA to CP, and a Carleton University Deans Summer Research Internship to SMH. The Ottawa Wavemakers (Hub Ottawa and WWF), Evergreen Freshwater Grant Program and Muskoka Brewery, TD Friends of Environment Foundation, and The 5 Gyres Institute provided support to MM.
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Information
Published In
FACETS
Volume 2 • Number 1 • January 2017
Pages: 301 - 314
Editor: Daniel E. Schindler
History
Received: 28 November 2016
Accepted: 26 January 2017
Version of record online: 21 March 2017
Copyright
© 2017 Vermaire et al. This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Data Availability Statement
All relevant data are within the paper.
Key Words
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Plain Language Summary
Microplastic pollution in Canada’s capital region
Authors
Author Contributions
All conceived and designed the study.
All performed the experiments/collected the data.
JCV and MM analyzed and interpreted the data.
JCV and MM contributed resources.
JCV, CP, and MM drafted or revised the manuscript.
Competing Interests
The authors have declared that no competing interests exist.
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Citations
Cite As
Jesse C. Vermaire, Carrington Pomeroy, Sofia M. Herczegh, Owen Haggart, and Meaghan Murphy. 2017. Microplastic abundance and distribution in the open water and sediment of the Ottawa River, Canada, and its tributaries. FACETS.
2: 301-314.
https://doi.org/10.1139/facets-2016-0070
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