Spirulina platensis ameliorates arsenic-mediated uterine damage and ovarian steroidogenic disorder
Abstract
Novel, non-invasive, painless oral therapeutic agents are needed to replace the painful conventional treatment of arsenic-associated health hazards with metal chelators. Our aim was to examine the effect of spirulina (Spirulina platensis (Geitler, 1925)) on arsenic-mediated uterine toxicity. Female Wistar rats were divided equally into four experimental treatment groups: control group, sodium arsenite group (1.0 mg/100 g body mass), spirulina placebo group (20 mg/100 g body mass), and sodium arsenite + spirulina group. In contrast with the control group, spectrophotometric and electrozymographic evaluation revealed that rats that ingested arsenic for 8 d showed significant diminution of the activities of superoxide dismutase, catalase, and peroxidase (p < 0.001). Mutagenic uterine DNA breakage and tissue damage were prominent following arsenic consumption by the rats. Oral delivery of spirulina resulted in a significant amelioration of arsenic-induced adverse oxidative stress and genotoxic state of rats. A significant low-signaling (p < 0.001) of gonadotropins and estradiol was also noted in the arsenic-treated rats, which was terminated by spirulina; this arsenic-primed adverse effect was significant (p < 0.05, p < 0.01). The spirulina treatment mechanism could be associated with augmentation of the antioxidant defense system that protects the arsenic-mediated pathological state of the uterus.
Introduction
Humans are exposed to arsenic through food, drinking water, and soil (Karagas et al. 1998). Arsenic poisoning is known to cause damage to several organs and tissues (Kurttio et al. 1999). Chronic arsenic exposure has also been linked to a significantly higher risk of cancer of the lung, liver, bladder, kidney, and colon (Smith et al. 2009). Many studies have shown that arsenic ingestion for a long period results in cutaneous lesions, hyperkeratosis, necrotic pigmentation, and melanosis (Fatmi et al. 2009). Arsenic causes chromosomal aberrations, sister chromatid exchange, and the alteration of DNA-methylation (Zhao et al. 1997). Long term exposure of arsenic in animal models has established male and female reproductive hazards: the alteration of ovarian and testicular steroidogenesis, the disruption of ovarian and testicular tissue, and the arrestation of spermatogenesis and folliculogenesis (Shukla and Pandey 1984; Chattopadhyay and Ghosh 2010). Arsenic-contaminated drinking water has xenoestrogenic effects on the anterior pituitary gland, and anterior pituitary hormone secretion is affected by arsenic (Ronchetti et al. 2016). Chronic environmental arsenic exposure in humans has been reported to cause reproductive disturbances in women (Zadorozhnaja et al. 2000) including critical pregnancy, low birth weight, reduced lactation, and impetuous abortion (Ahmad et al. 2001). Sodium arsenite causes slow activity of the hypothalamic-pituitary-gonadal axis and suppresses natural folliculogenesis by downregulating the plasma follicle stimulating hormone (FSH) (Chattopadhyay and Ghosh 2010). Arsenic ingestion for a long duration in humans elevates necrotic toxicity markers like lactate dehydrogenase (LDH) (Karim et al. 2010).
The invasive arsenic chelators British anti-Lewisite (BAL) and dimercaptosulfonic acid (DMSA) are frequently used for the removal of arsenic from the body. However, long term use of this treatment strategy is problematic because of the complexity of the treatment procedure as well as the moderate to severe side effects of these chelators (Inns et al. 1990). The detoxification of arsenic is a challenging issue, and investigators are now focusing on the use of different antioxidants, natural products, and plant components. All-trans retinoic acid (ATRA) relieved sodium-arsenite-induced reactive oxygen species (ROS) generation, restored redox balance, and prevented apoptosis (Chatterjee and Chatterji 2017). Vitamin E and selenium conjointly or alone ameliorated arsenic-induced cardiotoxicity (Bhattacharjee and Pal 2014) and female reprotoxicity (Chattopadhyay et al. 2000).
Recently, a microscopic blue-green alga (cyanobacterium), spirulina (Spirulina platensis (Geitler 1925)), is showing evidence of therapeutic importance, as it appears to reduce nephrotoxic substances in the body (Khan et al. 2006). Phycocyanin and the natural carotene in spirulina could potentially inhibit cancer cell development and growth (Peto et al. 1981; Shekelle et al. 1981). C-phycocyanin was reported as a potentially allergenic protein (Le et al. 2014) in spirulina-mediated anaphylaxis; however, preclinical studies related to acute and chronic toxicity, teratogenicity, fertility, and reproductive function assessments have demonstrated that spirulina itself is not toxic (Chamorro et al. 2002). Spirulina has also been listed as “generally recognized as safe” by the US Food and Drug Administration (Belay 2008).
Studies have shown that the antioxidant and anti-inflammatory effects of spirulina assist in the treatment of diabetes, hypertension, and hyperlipidaemia (Lee et al. 2008). Spirulina effectively promotes cell rejuvenation and, thereby, repairs liver and kidney tissue damage (Khan et al. 2001). In addition, spirulina has a protective effect against arsenic toxicity in humans (Misbahuddin et al. 2006; Rahman et al. 2008). Therefore, the aim of our investigation was to elucidate the role of the non-invasive oral application of spirulina in the amelioration of high dose arsenic-mediated uterine degeneration.
Materials and methods
Animal selection and care
In this experimental study, adult female albino rats (80–100 g body mass) were purchased from the approved animal provider Saha Enterprise, Kolkata, West Bengal. We conducted this study on Wistar strain adult female albino rats. The study was conducted with the approval of the Vidyasagar University’s ethical committee (ethical clearance No. IEC/7-2/C-2/16, dated 26 August 2016) and all experimental procedures were done according to the standards of The Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), India guidelines. The rats were reared in polycarbonate cages in the institutional animal house. They were adapted for 10 d in a room with a 12 h light:dark cycle, with 32 ± 2 °C temperatures and 50%–70% humidity. A standard pellet diet (Hindustan Lever Ltd., Mumbai, India) and water ad libitum were provided to the rats.
Treatment, study of estrous cycle, and sample collection
Twenty-four rats were equally and randomly separated into four groups of six. The vehicle-treated control group received only distilled water, the arsenic-treated group received 1.0 mg of sodium arsenite/100 g body mass, the spirulina placebo group received 20 mg of spirulina/100 g body mass, and the arsenic + spirulina treatment group received both 1.0 mg of sodium arsenite/100 g body mass and 20 mg spirulina/100 g body mass. In this experiment, 1.0 mg of sodium arsenite was dissolved in 0.1 mL of distilled water and applied per 100 g body mass of the rats. Spirulina tablets (500 mg, West-Coast Pharmaceutical, Ahmedabad, Gujarat) were crushed to produce spirulina dust, and 20 mg of spirulina were dissolved in 0.15 mL of sterile distilled water per 100 g body mass of the rats fresh daily before each use. Rats were treated via oral gavage for 8 d. The body masses of all rats were recorded throughout the treatment. The pattern of the estrous cycle was observed. Vaginal smear was collected daily from all animals. The feeding habits of all rats were observed throughout the treatment. On day nine the final body masses were recorded. Wistar rats were anesthetized with ketamine, and organs were collected from the anesthetized Wistar female rats following the standard protocol of Vidyasagar University’s ethical guidelines (ethical clearance No. IEC/7-2/C-2/16, dated 26 August 2016). Blood was collected for the separation of serum and then the animals were euthanized with CO2 as per the CPSCEA guideline. Dissected organs were preserved at − 20 °C until use.
Study of the estrous cycle
The different stages of the estrous cycle were studied in all rats following the collection of vaginal smears. The collected vaginal fluid was uniformly spread on a glass slide. The glass slides were allowed to dry for a few minutes and then were stained with Leishman stain and the phase of the estrous cycle was identified using a microscope (100× magnification).
Estimation of malondialdehyde (MDA) and conjugated dienes (CD) levels
Uterine tissues were homogenized (20% w/v) in ice-cold phosphate buffer (0.1 mol/L, pH 7.4) and centrifuged at 15 000g at 4 °C for 3 min. Supernatant was collected for the estimation of MDA and CD.
MDA levels were determined from the reaction of thiobarbituric acid with MDA. The amount of MDA formed was measured (Devasagayam et al. 2003) by taking the absorbance at 530 nm (ε = 1.56 × 105 L/mol·cm).
Conjugated dienes levels were determined using a standard method. The lipids were extracted with chloroform–methanol (2:1) and centrifuged at 1000g for 5 min. The residue of the lipid was then dissolved in 1.5 mL of cyclohexane. The hydroperoxide formed was measured at 233 nm (Kumar 2012).
Assay of super oxide dismutase (SOD), catalase, peroxidase, and lactate dehydrogenase (LDH) activities
Uterine tissues were homogenized in 100 mmol/L Tris–HCl (pH 7.4) to make a tissue concentration of 10% w/v and centrifuged at 10 000g for 20 min at 4 °C. A 100 μL portion of the tissue supernatant was mixed with 800 μL of triethanolamine-diethanolamine–HCl buffer (TDB) (Merck & Company, Inc., Kenilworth, New Jersey, USA), 40 μL of 7.5 mmol/L nicotinamide adenine dinucleotide phosphate (NADPH) (Sigma-Aldrich Corporation, St. Louis, Missouri, USA), and 25 μL of ethylenediaminetetraacetic acid (EDTA)-MnCl2 (Himedia, Mumbai, India). The activity of SOD was monitored at 340 nm from the rate of oxidation of the NADPH (Pattichis et al. 1994).
The activity of the catalase was measured colorimetrically. Uterine tissue was homogenized with ice-cold phosphate-buffered saline (PBS) (0.1 mol/L, pH 7.4) and centrifuged at 10 000g for 20 min at 4 °C. Here, dichromate in acetic acid was transformed first into perchromic acid and then into chromic acetate when heated in the presence of H2O2. The resulting chromic acetate was measured at 570 nm (Hadwan 2016). The catalase preparation permitted the splitting of H2O2 for different durations. The reaction was terminated at different time intervals by the addition of a dichromate–acetic acid mixture. One unit of activity was identified as one mole of H2O2 consumed per minute per milligram of protein.
For the peroxidase assay, uterine tissue was homogenized using PBS (0.1 mol/L, pH 7.0). A cocktail of 20 mmol/L of guiacol and 0.1 mL of supernatant was used in the presence of H2O2 (12.3 mmol/L) as a substrate. The time when the absorbance (436 nm) increased by 0.1 unit (Sadasivan and Manickam 1966) was noted.
The LDH serum levels were measured using LDH (P-L) kits, following the manufacturer’s (Tulip Group, Goa, India) instructions. LDH reduces pyruvate in the presence of NADH and thereby forms NAD. The rate of oxidation of NADH to NAD was determined by a decrease in the absorbance, which was proportional to the LDH activity.
Assessment of SOD, catalase, peroxidase, and LDH in native gel
Uterine tissues were homogenized with ice-cold PBS (1.0 mol/L, pH 7.4) and centrifuged at 10 000g for 20 min at 4 °C. The 40 μg of proteins from the tissue extract were applied to 12% polyacrylamide gel electrophoresis (PAGE). The gel was incubated with 2.3 mmol/L nitroblue tetrazolium (NBT), 28 μmol/L riboflavin, and 28 mmol/L tetramethylethylenediamine (TEMED) for 20 min in dark conditions. Upon light exposure the achromatic bands of SOD were visualized against a dark blue background (Weydert and Cullen 2010).
Uterine tissue extract containing 50 μg of proteins was electrophoresed on 8% PAGE for catalase detection. Gels were kept in 0.003% H2O2 solution for 10 min and stained with 2% potassium ferricyanide and 2% ferric chloride. Bluish-yellow bands appeared against a blue and green background (Lewis et al. 2005).
The 50 μg of proteins present in the tissue extract were loaded in 8% native gel and the gel was run with a power supply of 40 mA. The gel was then stained with 100 mg of benzidine powder in the presence of 4.5 mL glacial acetic acid and 30% H2O2 to observe the peroxidase band (Hasan and Aburahma 2014).
Electrozymography of LDH was performed in 8% agarose gel in 50 mmol/L Tris–HCl (pH 8.2) and 20 μL of serum were loaded into the different gel slots. The gel was electrophoresed at 170 V. The gel was incubated at 37 °C in the presence of Na-lactate, NAD and 1.0 mol/L Tris to develop the colour reaction in association with NBT and phenazine methosulphate. Finally, the gel was rinsed with water under light (Brandt et al. 1987).
DNA fragmentation analysis
Uterine tissues were used for DNA preparation and treated with 500 μL lysis buffer (50 mmol/L Tris-pH 8.0, 20 mmol/L EDTA, 10 mmol/L NaCl, 1% SDS, 0.5 mg/mL proteinase K) for 15 min at 4 °C. Centrifugation was performed at 12 000 rpm for 20 min. The collected supernatant was added to a 1:1 mixture of phenol:chloroform, gently agitated for 5 min, then precipitated into two parts of cold ethanol and one tenth part of sodium acetate (Paoletti et al. 1990). After spinning down and decantation, the precipitate was resuspended in 30 μL of deionized water–RNase solution (0.4 mL water + 5 μL of RNase) and 5 μL of loading buffer for 30 min at 37 °C. The 8.0% agarose gel with ethidium bromide was electrophoresed at 65 V and documented in a gel documentation system (Garcia-Martinez et al. 1993).
Comet assay
Slides were dipped into a 1% hot agarose solution. The uterine cell suspension was exposed to low melting point 0.6% agarose in PBS at 37 °C, fixed onto a glass slide precoated with 1% agarose, and a cover-slip was placed on the slide. A slight modification of the method described by Singh et al. (1988) was followed for the comet assay. Electrophoresis was performed for 30 min at 25 V and the current was adjusted to 300 mA by raising the buffer level. Slides were then neutralized with PBS and stained with a solution of 10 mg/mL ethidium bromide for 5 min. Excess stain was removed by washing with water. The slides were observed under a fluorescence microscope (Eclipse LV100 POL, Nikon, Tokyo, Japan) using Vis Comet software (Impuls Bildanalyse, Amsterdam, The Netherlands).
Serum hormone analysis
Serum levels of leutinizing hormone (LH) (Cat. No. ER1123), follicle stimulating hormone (FSH) (Cat. No. ER0960), and estradiol (Cat. No. ER1507) were measured using enzyme-linked immunosorbent assay (ELISA) kits following the procedures recommended by the manufacturer (Wuhan Fine Test, China).
Histo-architecture of ovary and uterus
Ovarian and uterine tissues were embedded in paraffin and sectioned into 5 μm slices, and stained with hematoxylin (Harris) and eosin (HE). Stained samples were observed under a microscope (CX21i, magnification 100× , Olympus, Tokyo, Japan).
Statistical analysis
In this experiment, data were expressed as mean ± SE, with n = 6 for each of the four experimental treatment groups (N = 24). Data from the supplemented group were compared with the control by analysis of variance (ANOVA) followed by post-hoc Dunnett’s t test and post-hoc least significant difference (LSD) test. p < 0.05 was considered a minimum level of significance.
Results
Feeding status, clinical signs, and body and organ masses of the animals
A normal diet was given to all study animals during the experiment. The animals in the arsenic-treated group showed significantly higher (p < 0.01) water consumption than the vehicle-treated control and arsenic + spirulina groups. A normal growth pattern was observed throughout the experimental schedule in all groups (Table 1). No significant changes in the body masses of the animals were observed throughout the experiment. The masses of the ovaries and uteri were expressed as a percentage of the body mass (ovarian and uterine somatic indices). A significant wet mass loss in the uterus was observed in the arsenic-treated group (p < 0.05) (Table 1). Less activity was noted in the arsenic-treated rats compared with the other treatment groups.
Table 1.
| Body mass (g) | Organo-somatic indices (g) | ||||
|---|---|---|---|---|---|
| Treatment | Initial | Final | Ovary in pair | Uterus | Water intake (mL) |
| Control | 95 ± 5.70 | 104 ± 5.78 | 0.057 ± 0.003 | 0.156 ± 0.037 | 9.5 ± 0.52 |
| Arsenic | 90 ± 8.06 | 109 ± 4.21 | 0.043 ± 0.007 | 0.104 ± 0.017a | 13.23 ± 0.8b |
| Spirulina | 91 ± 10.03 | 105 ± 9.06 | 0.054 ± 0.002 | 0.142 ± 0.013 | 9.94 ± 0.62 |
| Arsenic + spirulina | 88 ± 8.15 | 107 ± 9.56 | 0.052 ± 0.010 | 0.136 ± 0.007 | 10.38 ± 0.74 |
Note: The rats in the control group were provided with water. Doses of sodium arsenite (1.0 mg/100 g body mass per day) and spirulina (20 mg/100 g body mass) were given to the rats as per the treatment group (arsenic, spirulina, and arsenic + spirulina) for 8 d. The animals in the arsenic-treated group had significantly higher water consumption than the vehicle-treated control and co-administered arsenic + spirulina treatment groups. The data are presented as mean ± SE, n = 6.
a
Analysis of variance (ANOVA) followed by post-hoc Dunnett’s t test were used to determine the statistical significance at p < 0.05.
b
ANOVA followed by post-hoc Dunnett’s t test were used to determine the statistical significance at p < 0.01.
Pattern of the estrous cycle
The control group showed a regular estrous cycle pattern throughout the duration of the experiment. The regularity of the estrous cycle pattern ceased and persistent diestrus was observed in the arsenic-treated rats after 3–4 d of treatment. In contrast, the spirulina-supplemented arsenic-treated rats showed a more regular pattern (Fig. 1).
Fig. 1.
Status of oxidative stress markers and LDH
There was a significant increase in the uterine MDA and CD in the arsenic-treated group (p < 0.001) compared with the control group (Fig. 2). However, co-treatment with spirulina corrected this alteration of the lipid peroxidation end products in the uteri of arsenic-treated rats.
Fig. 2.
There was a significant decrease in the uterine SOD, catalase, and peroxidase activities in the arsenic-treated group (p < 0.001) compared with the control group. Interestingly, a restoration of SOD, catalase, and peroxidase activities was observed in the arsenic + spirulina treatment group (Fig. 2). There was a significant increase in serum LDH activity in the arsenic-treated group (p < 0.001) compared with the control group. Co-administration of arsenic + spirulina caused a significant recovery in serum LDH activity (p < 0.05, p < 0.01) compared with the that in the arsenic-treated rats (Fig. 2).
We further evaluated the enzymatic antioxidants electrozymographically. The results showed a significant decrease in uterine SOD, catalase, and peroxidase activities (Fig. 3) in arsenic-treated rats compared with the control group. The electrozymograms showed that the band intensities of these three enzymatic antioxidants were reduced in arsenic-treated rats compared with the vehicle-treated control group (Figs. 3A–3C). Co-administration of arsenic + spirulina reversed the condition compared with the arsenic-treated rats, followed by the appearance of a more intense band.
Fig. 3.
Electrozymography of serum lactate dehydrogenase (LDH) was performed to assess the necrotic risk factor (Fig. 3D). The arsenic-treated group had a more conspicuous band than the control group, whereas the arsenic + spirulina treatment group showed a very faint or nearly imperceptible band. This release of the enzyme was significantly reduced in rats treated with arsenic + spirulina as demonstrated by the weak expression of this necrotic marker.
Serum gonadotrophins and ovarian steroidogenesis
A significant downregulation of LH, FSH, and estradiol was observed following 8 d of arsenic ingestion (p < 0.001) compared with the control group (Fig. 4). Co-administration of arsenic + spirulina significantly decreased the inhibitory influence of arsenic on the signaling of these hormones. The co-administration of arsenic + spirulina caused a significant increase (p < 0.05, p < 0.01) in serum gonadotrophins and ovarian steroidogenic activity when compared with arsenic-treated rats (Fig. 4).
Fig. 4.
Histopathology and uterine DNA
The DNA agarose gel electrophoresis in vivo model demonstrated that arsenic (AsIII) impaired uterine DNA (Fig. 5A1). The uterine DNA degradation was more prominent in the arsenic-treated group (Fig. 5A1; Lane 2) than in the unexposed rats (Fig. 5A1; Lane 1). The arsenic + spirulina co-treatment (Fig. 5A1; Lane 3, 4) partially, but nonetheless significantly, reduced the degradation of DNA, which was further supported by the relative density versus relative migration (Fig. 5A2). Furthermore, significant comet numbers along with increased tail length were visible from the single cell DNA study in the arsenic-treated group and lower comet and tail length were shown in rats treated with arsenic + spirulina (p < 0.001) (Fig. 5B; Table 2).
Fig. 5.
Table 2.
| Control | Arsenic | Spirulina | Arsenic + spirulina | |
|---|---|---|---|---|
| Comet in number | 1.17 ± 0.307 | 6.167 ± 0.542a | 1.5 ± 0.22 | 2.0 ± 0.365 |
| Comet tail length (μm) | 4.375 ± 0.54 | 10.494 ± 1.019a | 3.897 ± 0.342 | 4.104 ± 0.549 |
Note: The data are presented as mean ± SE, n = 6.
a
Analysis of variance (ANOVA) followed by post-hoc Dunnett’s t test were used to determine the statistical significance at p < 0.001.
HE-stained uterine sections demonstrated a remarkable loss of uterine secretory glands in arsenic-treated rats. A distinct thinning of the uterine perimetrium, myometrium, and endometrium was well documented in rats exposed to sodium arsenite. Spirulina consumption in arsenic-treated rats resulted in the partial, but significant, thickening of the layers towards the levels of the control group (Fig. 6A). Renewal of the uterine secretory glands was also achieved following co-treatment with arsenic + spirulina. A noticeable decline in the the primary classes of ovarian preantral and antral follicles was observed in arsenic-treated rats. A higher degree of follicular regression and follicular atresia with a significant reduction in the numbers of graafian follicles were also documented in arsenicated rats (Fig. 6B). Co-administration of arsenic + spirulina significantly reversed the above effects as demonstrated by the reappearance of mature follicles and smaller numbers of regressing follicles (Fig. 6B).
Fig. 6.
Discussion
A trivalent form of arsenic (AsIII) is recognized for its high degree of toxicity. Previously, a comparatively low dose of arsenic for 28 d (Chattopadhyay et al. 2012; Acharyya et al. 2015) was considered to demonstrate the toxic effect of arsenic on different body parts. Our study used a comparatively higher dose of arsenic (10 mg/kg body mass) with 8 d of treatment to evaluate the effects of arsenic toxicity in female reproductive organs. Our study also focused on whether spirulina is effective against such a high dose of arsenic. However, arsenic at a dose of 10 mg/kg body mass for a short duration exhibited a significant level of free radical generation in the organs (Manna et al. 2007; Sinha et al. 2008; Bhattacharya and Haldar 2013). Arsenic treatment at a dose of 10 mg/kg body mass for a short duration like 8 d was previously used by our group (Maity et al. 2018). In this experiment, arsenic (AsIII) was involved in the generation of oxidative stress and manifested in elevated uterine MDA and CD levels (Fig. 2). This indicates that the trivalent form of arsenic yields reactive oxygen species in association with H2O2 and, therefore, produces end products in the form of lipid peroxides and conjugated dienes (Wang and Xu 2006). Electrozymograms of SOD in our investigation revealed a decreased intensity of SOD bands in the arsenic-treated group (Fig. 3A). The activity of SOD in the gel was shown to be suppressed by arsenic after staining with NBT. Rana et al. (2012) also documented that arsenic reduced mRNA expression of the SOD gene. Furthermore, in the current study we electrozymographically documented decreased catalase and peroxidase activity indicated by the existence of weak bands following arsenic ingestion (Figs. 3B and 3C). These results indicate the possible impairment of H2O2 detoxification in the uterine tissue due to the diminished activity of catalase. Previous studies have shown that the inhibition of catalase by arsenic trioxide resulted in intracellular ROS accumulation (Wang et al. 2012). This impairment in catalase activity by arsenic may be mediated via the modulation of its expression at the level of mRNA transcription (Wang et al. 2012). Likewise, our result revealed that the expression of peroxidase was also reduced in response to arsenic. This finding strongly suggests that H2O2 accumulates during programmed cell death (Weydert and Cullen 2010).
LDH, a necrotic biomarker was also considered and evaluated in this investigation (Kim and Dang 2006; Zhang et al. 2015). Serum LDH expression was noticeably elevated in the arsenic-treated group (Fig. 3D). This elevation may play a critical role in the fibrotic changes of the organs by stimulating collagen deposition (Corsini et al. 1994; Kottmann et al. 2012; Leppert et al. 2014).
Arsenic exposure had detrimental effects on plasma levels of LH, FSH, and estradiol (Fig. 4) along with low ovarian and uterine mass (Fig. 4). Rats remained in consistent diestrus or metestrus after 4 d of treatment compared with the control rats, whereas synchronization of the estrous cycle was achieved following the treatment with spirulina. Consistent diestrus in arsenic-treated rats after 5 d may be due to low estrogen plasma levels (Parshad et al. 1989). This indicates that arsenic indirectly imposes its effect on ovaries by altering the regulation of the pituitary–ovarian axis. It is possible that the deregulation of the gonadotrophins from arsenic may result in low ovarian mass and follicular regression (Tagatz et al. 1970; Kulin and Reiter 1973), whereas inhibition of the estradiol signalling in these rats may be the limiting factor causing decreased uterine mass and the degradation of the normal uterine histo-architecture (Edman 1983).
Spirulina co-treatment significantly mitigated arsenic-induced uterine oxidative stress and ovarian disorders. Spirulina is a non-enzymatic antioxidant and may, thereby, improve the enzymatic antioxidant status. The cellular degeneration from arsenic exposure was likely necrotic in nature, which can be demonstrated by uterine DNA fragmentation (Fig. 5A1). In this study, arsenic showed conspicuous DNA fragmentation (Fig. 5A1 Lane 2) in the uterine tissues compared with the control (Fig. 5A1 Lane 1). The formation of ROS by trivalent arsenicals may ultimately lead to DNA damage, thereby initiating a suppression of DNA repair systems and the repair of oxidative DNA damage (Kligerman et al. 2003). In this experiment, the apoptotic features were evident from the comet assay and DNA fragmentation image (Figs. 5B and 5A1). This study demonstrated that co-treatment with arsenic + spirulina (Fig. 5A1 Lane 3, 4) partially, but significantly, reduced the degradation of DNA caused by the arsenic. The protection of DNA by spirulina against arsenite-induced toxicity was also demonstrated in single cell DNA damage (Fig. 5B; Table 2). A low serum LDH and the protection of DNA damage following arsenic + spirulina co-administration in rats may act as the barrier to necrotic progression (Ding et al. 2005). This overall defensive mechanism of spirulina might have terminated arsenic-primed inducement of apoptosis and protected uterine and ovarian tissues from necrotic damage, as there was an improvement in the ovarian–uterine histo-architecture (Figs. 6A and 6B). This may be connected with the upregulation of gonadotrophin release and ovarian steroidogenesis (Tagatz et al. 1970; Kulin and Reiter 1973).
Other investigators have explored the therapeutic effects of spirulina on arsenic-induced male reproductive toxicity (Bashandy et al. 2015). Spirulina is a unique alga because it is enriched in essential amino acids such as sulfolipids, linolenic acid, protein, essential fatty acids, vitamins, flavonoids, and minerals (Mendes et al. 2003; Dartsch 2008). In addition, spirulina contains phycocyanin and phycocyanobilin (McCarty 2013), which have been claimed to inhibit peroxyl-radical-induced lipid peroxidation and NADPH-dependent superoxide production (Bhat and Madyastha 2001; Zheng et al. 2013). Because it has the capacity to bind with heavy metals, spirulina may demonstrate a chelating property to trap arsenic (Plazinski 2013). Methylation of arsenic in association with S-adenosylmethionine involves methyl cobalamin (CH3B12) and reduced glutathione for arsenic clearance and detoxification with the involvement of enzymes (Nakamura 2011). Arsenite S-adenosyl methionine methyltransferase was isolated and characterized from spirulina (Guo et al. 2016). An in vitro assay system demonstrated the arsenic methylation activity of spirulina (Guo et al. 2016). This methylation process is the essential prerequisite for the removal of arsenic. Therefore, spirulina may eventually promote arsenic methylation and detoxification.
Conclusion
Co-treatment with arsenic + spirulina in rats may protect the uterus and ovaries from arsenic-induced reproductive pathogenesis because of spirulina acting as a free radical scavenger, thereby limiting the oxidative stress. Secondly, spirulina may improve the extent of methylation in the detoxification of arsenic and, thereby, safeguard the normal morphology of the uterus and ovaries. Further extensive study of spirulina is necessary to fully elucidate the underlying mechanism of action.
Acknowledgement
This research was partly supported by a grant from UGC Maulana Azad National Fellowship (No. F1-17.1/2014-15/MANF-2014-15-MUS-WES-36088).
References
Acharyya N, Deb B, Chattopadhyay S, and Maiti S. 2015. Arsenic-induced antioxidant depletion, oxidative DNA breakage, and tissue damages are prevented by the combined action of folate and vitamin B12. Biological Trace Element Research, 168(1): 122–132.
Ahmad SA, Sayed MHSU, Barua S, Khan MH, Faruquee MH, Jalil A, et al. 2001. Arsenic in drinking water and pregnancy outcomes. Environmental Health Perspectives, 109(6): 629–631.
Bashandy SAE, Awdan SAE, Ebaid H, and Alhazza IM. 2015. Antioxidant potential of Spirulina platensis mitigates oxidative stress and reprotoxicity induced by sodium arsenite in male rats. Oxidative Medicine and Cellular Longevity, 2016(2016): 7174351.
Belay A. 2008. Spirulina (Arthrospira): production and quality assurance. In Spirulina in human nutrition and health. Edited by ME Gershwin and A Belay. CRC Press, New York, New York. pp. 1–25.
Bhat BV, and Madyastha KM. 2001. Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: protection against oxidative damage to DNA. Biochemical and Biophysical Research Communications, 285(2): 262–266.
Bhattacharjee S, and Pal S. 2014. Additive protective effects of selenium and vitamin E against arsenic induced lipidemic and cardiotoxic effects in mice. International Journal of Pharmacy and Pharmaceutical Sciences, 6(5): 406–413.
Bhattacharya S, and Haldar PK. 2013. Trichosanthes dioica root alleviates arsenic induced myocardial toxicity in rats. Journal of Environmental Pathology, Toxicology and Oncology, 32(3): 251–261.
Brandt RB, Laux JE, Spainhour SE, and Kline ES. 1987. Lactate dehydrogenase in rat mitochondria. Archives of Biochemistry and Biophysics, 259(2): 412–422.
Chamorro G, Salazar M, Araujo KG, dos Santos CP, Ceballos G, and Castillo LF. 2002. Update on the pharmacology of Spirulina (Arthrospira), an unconventional food. Archivos Latinoamericanos de Nutricion, 52: 232–240.
Chatterjee A, and Chatterji U. 2017. All-trans retinoic acid ameliorates arsenic-induced oxidative stress and apoptosis in the rat uterus by modulating MAPK signaling proteins. Journal of Cellular Biochemistry, 118: 3796–3809.
Chattopadhyay S, and Ghosh D. 2010. The involvement of hypophyseal-gonadal and hypophyseal-adrenal axes in arsenic-mediated ovarian and uterine toxicity: modulation by hCG. Journal of Biochemical and Molecular Toxicology, 24(1): 29–41.
Chattopadhyay S, Misro M, Debnath J, and Ghosh D. 2000. Supplementary effect of α-tocopherol succinate (vitamin E) on sodium arsenite-induced ovarian steroidogenic function and plasma levels of gonadotrophins in mature albino rats. Toxic Substance Mechanisms, 19: 137–150.
Chattopadhyay S, Deb B, and Maiti S. 2012. Hepatoprotective role of vitamin B12 and folic acid in arsenic intoxicated rats. Drug and Chemical Toxicology, 35(1): 81–88.
Corsini E, Luster MI, Mahler J, Craig WA, Blazka ME, and Rosenthal GJ. 1994. A protective role for T lymphocytes in asbestos-induced pulmonary inflammation and collagen deposition. American Journal of Respiratory Cell and Molecular Biology, 11(5): 531–539.
Dartsch PC. 2008. Antioxidant potential of selected Spirulina platensis preparations. Phytotherapy Research, 22(5): 627–633.
Devasagayam TPA, Boloor KK, and Ramasarma T. 2003. Methods for estimating lipid peroxidation: an analysis of merits and demerits. Indian Journal of Biochemistry & Biophysics, 40(5): 300–308.
Ding W, Hudson LG, and Liu KJ. 2005. Inorganic arsenic compounds cause oxidative damage to DNA and protein by inducing ROS and RNS generation in human keratinocytes. Molecular and Cellular Biochemistry, 279(1–2): 105–112.
Edman CD. 1983. The effects of steroids on the endometrium. Seminars in Reproductive Medicine, 1: 179–187.
Fatmi Z, Azam I, Ahmed F, Kazi A, Gill AB, Kadir MM, et al. 2009. Health burden of skin lesions at low arsenic exposure through groundwater in Pakistan. Is river the source? Environmental Research, 109(5): 575–581.
Garcia-Martinez V, Macias D, Ganan Y, Garcia-Lobo JM, Francia MV, Fernandez-Teran MA, et al. 1993. Internucleosomal DNA fragmentation and programmed cell death (apoptosis) in the interdigital tissue of the embryonic chick leg bud. Journal of Cell Science, 106: 201–208.
Geitler L. 1925. Cyanophyceae. In Die Süsswasser-Flora Deutschlands, Österreichs und der Schweiz. Edited by A Pascher. Gustav Fischer, Jena, Germany. Vol. 12, pp. 1–450.
Guo Y, Xue X, Yan Y, Zhu Y, Yang G, and Ye J. 2016. Arsenic methylation by an arsenite S-adenosyl methioninemethyl transferase from Spirulina platensis. Journal of Environmental Sciences, 49: 162–168.
Hadwan MH. 2016. New method for assessment of serum catalase activity. Indian Journal of Science and Technology, 9(4): 1–5.
Hasan HR, and Aburahma NA. 2014. Electrophoresis profile of total peroxidases in saliva and sera of patients with different oral tumors. Oriental Journal of Chemistry, 30(1): 81–86.
Inns RH, Rice P, Bright JE, and Marrs TC. 1990. Evaluation of the efficacy of dimercapto chelating agents for the treatment of systemic organic arsenic poisoning in rabbits. Human & Experimental Toxicology, 9(4): 215–220.
Karagas MR, Tosteson TD, Blum J, Morris JS, Baron JA, and Klaue B. 1998. Design of an epidemiologic study of drinking water arsenic exposure and skin and bladder cancer risk in a U.S. population. Environmental Health Perspectives, 106(4): 1047–1050.
Karim MR, Salam KA, Hossain E, Islam K, Ali N, Haque A, et al. 2010. Interaction between chronic arsenic exposure via drinking water and plasma lactate dehydrogenase activity. Science of the Total Environment, 409(2): 278–283.
Khan M, Shobha JC, Mohan IK, Rao Naidu MU, Prayag A, Kutala VK, et al. 2006. Spirulina attenuates cyclosporine-induced nephrotoxicity in rats. Journal of Applied Toxicology, 26(5): 444–451.
Khan MAK, Choudhury SAR, Misbahuddin M, Islam AZMM, and Shahjahan M. 2001. Effects of Spirulina in the treatment of chronic arsenic poisoning in Bangladesh. Bangladesh Journal of Medical Science, 7: 223–231.
Kim J-W, and Dang CV. 2006. Cancer’s molecular sweet tooth and the Warburg effect. Cancer Research, 66(18): 8927–8930.
Kligerman AD, Doerr CL, Tennant AH, Harrington Brock K, Allen JW, Winkfield E, et al. 2003. Methylated trivalent arsenicals as candidate ultimate genotoxic forms of arsenic: induction of chromosomal mutations but not gene mutations. Environmental and Molecular Mutagenesis, 42(3): 192–205.
Kottmann RM, Kulkarni AA, Smolnycki KA, Lyda E, Dahanayake T, Salibi R, et al. 2012. Lactic acid is elevated in idiopathic pulmonary fibrosis and induces myofibroblast differentiation via pH-dependent activation of transforming growth factor-β. American Journal of Respiratory and Critical Care Medicine, 186(8): 740–751.
Kulin HE, and Reiter EO. 1973. Gonadotropins during childhood and adolescence: a review. Pediatrics, 51(2): 260–271.
Kumar A. 2012. Effect of simvastatin on paraoxonase 1 (PON1) activity and oxidative stress. In Significance of lipid profile assay as diagnostic and prognostic tool. Edited by A Kumar. CreateSpace Independent Publishing Platform, Santa Barbara, California. pp. 105–109.
Kurttio P, Pukkala E, Kahelin H, Auvinen A, and Pekkanen J. 1999. Arsenic concentrations in well water and risk of bladder and kidney cancer in Finland. Environmental Health Perspectives, 107(9): 705–710.
Le T-M, Knulst AC, and Rockmann H. 2014. Anaphylaxis to Spirulina confirmed by skin prick test with ingredients of Spirulina tablets. Food and Chemical Toxicology, 74: 309–310.
Lee EH, Park JE, Choi YJ, Huh KB, and Kim WY. 2008. A randomized study to establish the effects of Spirulina in type 2 diabetes mellitus patients. Nutrition Research and Practice, 2(4): 295–300.
Leppert PC, Jayes FL, and Segars JH. 2014. The extracellular matrix contributes to mechanotransduction in uterine fibroids. Obstetrics and Gynecology International, 2014: 783289.
Lewis A, Du J, Liu J, Ritchie JM, Oberley LW, and Cullen JJ. 2005. Metastatic progression of pancreatic cancer: changes in antioxidant enzymes and cell growth. Clinical & Experimental Metastasis, 22(7): 523–532.
Maity M, Perveen H, Dash M, Jana S, Khatun S, Dey A, et al. 2018. Arjunolic acid improves the serum level of vitamin B12 and folate in the process of the attenuation of arsenic induced uterine oxidative stress. Biological Trace Element Research, 182: 78–90.
Manna P, Sinha M, and Sil PC. 2007. Protection of arsenic-induced hepatic disorder by arjunolic acid. Basic & Clinical Pharmacology & Toxicology, 101: 333–338.
McCarty MF. 2013. Nutraceutical strategies for ameliorating the toxic effects of alcohol. Medical Hypotheses, 80(4): 456–462.
Mendes RL, Nombre BP, Cardoso MT, Pereira AP, and Palavra AF. 2003. Supercritical carbon dioxide extraction of compounds with pharmaceutical importance from microalgae. Inorganica Chimica Acta, 356: 328–334.
Misbahuddin M, Islam AZMM, Khandker S, Mahmud IA, Islam N, and Anjumanara IM. 2006. Efficacy of Spirulina extract plus zinc in patients of chronic arsenic poisoning: a randomized placebo-controlled study. Clinical Toxicology, 44(2): 135–141.
Nakamura K. 2011. Biomimetic and bio-inspired catalytic system for arsenic detoxification: bio-inspired catalysts with vitamin-B12 cofactor. In On biomimetics. Edited by L Pramatarova. IntechOpen, Rijeka, Croatia. pp. 213–228.
Paoletti F, Mocali A, and Aldinucci D. 1990. Superoxide-driven NAD(P)H oxidation induced by EDTA-manganese complex and mercaptoethanol. Chemico-Biological Interactions, 76(1): 3–18.
Parshad VR, Kaur P, and Guraya SS. 1989. Reproductive cycles of mammals: Rodentia. In Reproductive cycles of Indian vertebrates. Edited by SK Saidapur. Allied Publishers Ltd., Bombay, India. pp. 347–408.
Pattichis KI, Louca LL, and Glover V. 1994. Quantitation of soluble superoxide dismutase in rat striata, based on the inhibition of nitrite formation from hydroxylammonium chloride. Analytical Biochemistry, 221(2): 428–431.
Peto R, Doll R, and Buckly JD. 1981. Can dietary beta-carotene materially reduce human cancer rates? Nature, 290(5803): 201–208.
Plazinski W. 2013. Binding of heavy metals by algal biosorbents. Theoretical models of kinetics, equilibria and thermodynamics. Advances in Colloid and Interface Science, 197–198: 58–67.
Rahman MH, Islam AZMM, and Sikder MS. 2008. Dynamics of Spirulina in promoting health benefits for arsenicosis patients. Journal of Bangladesh College of Physicians and Surgeons, 26(1): 14–21.
Rana T, Bera AK, Das S, Bhattacharya D, Pan D, and Das SK. 2012. Metabolic adaptations to arsenic-induced oxidative stress in male wistar rats. Journal of Biochemical and Molecular Toxicology, 26(3): 109–116.
Ronchetti SA, Novack GV, Bianchi MS, Crocco MC, Duvilanski BH, and Cabilla JP. 2016. In vivo xenoestrogenic actions of cadmium and arsenic in anterior pituitary and uterus. Reproduction, 152: 1–10.
Sadasivan S, and Manickam A. 1966. Peroxidase. In Methods in biochemistry. Edited by S Sadasivan and A Manickam. New Age International Publishers, New Delhi, India. pp. 108–110.
Shekelle RB, Lepper M, Liu S, Maliza C, Raynor WJ Jr, Rossof AH, et al. 1981. Dietary vitamin A and risk of cancer in the Western Electric study. The Lancet, 318(8257): 1185–1190.
Shukla JP, and Pandey K. 1984. Impaired spermatogenesis in arsenic treated freshwater fish, Colisa fasciatus (Bl. and Sch.). Toxicology Letters, 21(2): 191–195.
Singh NP, McCoy MT, Tice RR, and Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1): 184–191.
Sinha M, Manna P, and Sil PC. 2008. Protective effect of arjunolic acid against arsenic-induced oxidative stress in mouse brain. Journal of Biochemical and Molecular Toxicology, 22: 15–26.
Smith AH, Ercumen A, Yuan Y, and Steinmaus CM. 2009. Increased lung cancer risks are similar whether arsenic is ingested or inhaled. Journal of Exposure Science & Environmental Epidemiology, 19(4): 343–348.
Tagatz GE, Fialkow PJ, and Smith D. 1970. Hypogonadotropic hypogonadism associated with anosmia in the female. The New England Journal of Medicine, 283(24): 1326–1329.
Wang L, and Xu ZR. 2006. Effects of arsenic (AsIII) on lipid peroxidation, glutathione content and antioxidant enzymes in growing pigs. Asian-Australasian Journal of Animal Sciences, 19(5): 727–733.
Wang Y, Wei Y, Zhang H, Shi Y, Li Y, and Li R. 2012. Arsenic trioxide induces apoptosis of p53 null osteosarcoma MG63 cells through the inhibition of catalase. Medical Oncology, 29(2): 1328–1334.
Weydert CJ, and Cullen JJ. 2010. Measurement of superoxide dismutase, catalase, and glutathione peroxidase in cultured cells and tissue. Nature Protocols, 5(1): 51–66.
Zadorozhnaja TD, Little RE, Miller RK, Mendel NA, Taylor RJ, Presley BJ, et al. 2000. Concentrations of arsenic, cadmium, copper, lead, mercury, and zinc in human placentas from two cities in Ukraine. Journal of Toxicology and Environmental Health, Part A, 61(4): 255–263.
Zhang J, Yao YH, Li BG, Yang Q, Zhang PY, and Wang HT. 2015. Prognostic value of pretreatment serum lactate dehydrogenase level in patients with solid tumors: a systematic review and meta-analysis. Scientific Reports, 5(5): 9800.
Zhao CQ, Young MR, Diwan BA, Coogan TP, and Waalkes MP. 1997. Association of arsenic-induced malignant transformation with DNA hypomethylation and aberrant gene expression. Proceedings of the National Academy of Sciences of the USA, 94(20): 10907–10912.
Zheng J, Inoguchi T, Sasaki S, Maeda Y, McCarty MF, Fujii M, et al. 2013. Phycocyanin and phycocyanobilin from Spirulina platensis protect against diabetic nephropathy by inhibiting oxidative stress. American Journal of Physiology—Regulatory, Integrative and Comparative Physiology, 304(2): R110–R120.
Information & Authors
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Published In
FACETS
Volume 3 • Number 1 • October 2018
Pages: 736 - 753
Editor: Peter Zahradka
History
Received: 11 July 2017
Accepted: 11 April 2018
Version of record online: 12 July 2018
Copyright
© 2018 Khatun et al. This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Data Availability Statement
All relevant data are within the paper.
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Author Contributions
SK and SC conceived and designed the study.
All performed the experiments/collected the data.
SK, MM, and SC analyzed and interpreted the data.
SK and SC contributed resources.
All drafted or revised the manuscript.
Competing Interests
The authors have declared that no competing interests exist.
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Shamima Khatun, Moulima Maity, Hasina Perveen, Moumita Dash, and Sandip Chattopadhyay. 2018. Spirulina platensis ameliorates arsenic-mediated uterine damage and ovarian steroidogenic disorder. FACETS.
3(1): 736-753. https://doi.org/10.1139/facets-2017-0099
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