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[Paper Type: Communication] AND [Subject Areas: Biological and Life Sciences] (5) | 24 Aug 2024 |
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- OPEN ACCESSDeadly outbreaks and illnesses due to Shiga toxin-producing Escherichia coli (STEC) occur worldwide; however, the cultivation methods required for adequate monitoring and traceback investigations are inefficient at best. Detection of STEC relies heavily on enrichment; yet no standard media or protocols exist. Furthermore, whether enrichment may bias detection of multiple STEC serogroups from complex samples is unknown. Thus, 14 STEC strains of serogroups O157 and the top six non-O157s (O26, O45, O103, O111, O121, and O145) were enriched in pairs for 6–78 h in broth and evaluated by quantitative polymerase chain reaction (qPCR). Here we show that a conventional 6-h enrichment protocol did not result in intra-species culture bias for the isolates tested. However, subsequent enrichments often produced biased cultures, with differences in the qPCR gene copy number ≥2 log10 apparent in 12%, 38%, and 52% of competitions after 30, 54, and 78 h of consecutive enrichments, respectively. Some strains were able to prevail and (or) out-compete the opponent strain in 100% of competitions. Our results suggest that culture bias should be considered and (or) evaluated further due to the potential implications during routine pathogen screening and outbreak investigations.
- OPEN ACCESS
- Emily M. Merlo,
- Kathryn A. Milligan,
- Nola B. Sheets,
- Christopher J. Neufeld,
- Tao M. Eastham,
- A.L. Ka’ala Estores-Pacheco,
- Dirk Steinke,
- Paul D.N. Hebert,
- Ángel Valdés, and
- Russell C. Wyeth
The mollusc nudibranch genus Hermissenda Bergh, 1879 was recently discovered to include three pseudocryptic species, dividing a single species H. crassicornis (sensu lato) into H. crassicornis Escholtz, 1831, H. opalescens J.G. Cooper, 1863, and H. emurai Baba, 1937. The species were distinguished by both genetic and morphological evidence, and the distribution of sampled animals suggested the three species had mostly distinct geographical ranges. Here, we report the presence of both H. crassicornis and H. opalescens in Barkley and Clayoquot Sounds, British Columbia, Canada, based on diagnostic characters and molecular data congruent with the differences described for these two species. This result extends the region of sympatry for the two species from northern California, USA, to, at least, Vancouver Island, British Columbia in 2016. Depending on how long this overlap has occurred, the possible northward expansion of H. opalescens would have implications for understanding the effects of short- or long-term environmental changes in ocean temperatures as well as complicating the interpretation of past neurobiological studies of H. crassicornis (sensu lato). - OPEN ACCESS
- Barry N. Madison,
- Jessie Reynolds,
- Lauren Halliwell,
- Tim Leshuk,
- Frank Gu,
- Kerry M. Peru,
- John V. Headley, and
- Diane M. Orihel
Our study evaluates the efficacy of a “green” (i.e., sustainable, recyclable, and reusable) technology to treat waste waters produced by Canada’s oil sands industry. We examined the ability of a novel advanced oxidative method—ultra-violet photocatalysis over titanium dioxide (TiO2)-coated microparticles—to reduce the toxicity of naphthenic acid fraction components (NAFC) to early life stages of the fathead minnow (Pimephales promelas). Lengthening the duration of photocatalysis resulted in greater removal of NAFC from bioassay exposure waters; low- and high-intensity treatments reduced NAFC concentrations to about 20 and 3 mg/L (by Fourier-transformed infrared spectroscopy, FTIR), respectively. Treatments reduced the acute lethality of NAFC to fathead minnows by over half after low-intensity treatment and three-fold after high-intensity treatment. However, incomplete degradation in low-intensity treatments increased the incidence of chronic toxicity relative to untreated NAFC solutions and cardiovascular abnormalities were common even with >80% of NAFC degraded. Our findings demonstrate that photocatalysis over TiO2 microparticles is a promising method for mitigating the toxicity of oil sands process-affected water-derived NAFC to fish native to the oil sands region, but the intensity of the photocatalytic treatment needs to be considered carefully to ensure adequate mineralization of toxic constituents. - OPEN ACCESS
- D.R. Ballard,
- A.J. Davis,
- R.B. Fuller,
- A.R. Garner,
- A.D. Mileham,
- J.D. Serna,
- D.E. Brue,
- C.M. Harding,
- C.D. Dodgen,
- W. Culpepper,
- B. Piatt,
- S.E. Rosario, and
- A.L.J. Duffus
Ranaviruses are globally emerging infections of poikilothermic vertebrates and belong to the viral family Iridoviridae. The six species of ranaviruses are responsible for unknown numbers of infections and disease and mortality events around the world in amphibians, fish, and reptiles. Genomic investigations have shown that there are 24 core genes shared by all iridoviruses. In this study, we examine the utility of each of these genes in reconstructing phylogenetic relationships across six species of Ranavirus. We also performed dot-plot analysis for the 17 isolates in the study. For large-scale differentiation, using the major capsid protein gene creates a tree similar to the whole genome tree. Other comparable genes include open reading frame (ORF) 19R (a serine–theonine protein kinase) and ORF 88R (Erv I/Alr Family protein). The poorest candidate for phylogenetic reconstruction, due to high homology, was ORF 1R (a putative replication factor and (or) DNA binding-packing protein). There are a plethora of genes that may be useful to examine phylogenies at smaller scales (e.g., to examine local adaptation); however, they do not necessarily belong to the set of highly conserved core genes. - OPEN ACCESSOpen access (OA) allows for peer-reviewed research to be freely accessed and there has been a collective shift from both researchers and publishers towards more OA publishing. OA typically occurs either through article-processing charges (the gold road) or via self-archiving (the green road); the former can be expensive, while the latter has seen minimal uptake. The gold road of OA has led to predatory publishers and, to some, questionable publications. Here, I used publicly available grant information in Canada and combined this with individual publishing statistics to test a variety of factors and their influence on OA publishing. I showed that an individual’s award amount, H-index, and gender did not influence the proportion of OA articles they published, but an individual’s H-index scaled with the number of OA publications. Institute size influenced OA publishing patterns, with researchers at large universities (i.e., >20 000 full-time students) publishing proportionately more OA articles than medium and small institutes. I discuss the potential for this pattern to build on pre-existing systemic biases when it comes to funding and publishing.