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  • OPEN ACCESS
    Wavelet transform as a postprocessing diagnostic tool for fault identifications of high-voltage direct current transmission system
    FACETS • Vol. 1 • pp. 17 - 26
    This short communication focuses on exploiting the inherent advantages of discrete wavelet transformation (DWT) as a diagnostic tool for post-processing and for identifying the faults that occur in the standard high-voltage direct-current (HVDC) transmission network. In particular, a set of investigations are developed and examined for single-line-to-ground fault on the generation and on the load side converter, and DC-link fault. For this purpose, a standard 12-pulse line-commutated converter (LCC)-HVDC transmission network along with the DWT algorithm is numerically modeled in the MATLAB/PLECS simulation software. Furthermore, in this paper, a set of designed faulty conditions are predicted using the output of DWT and the results of numerical simulation are presented. Results are in good agreement with expectations to prove that DWT is an effective tool for fault diagnostics.
  • OPEN ACCESS
    Competition during enrichment of pathogenic Escherichia coli may result in culture bias
    FACETS • Vol. 1 • pp. 114 - 126
    Deadly outbreaks and illnesses due to Shiga toxin-producing Escherichia coli (STEC) occur worldwide; however, the cultivation methods required for adequate monitoring and traceback investigations are inefficient at best. Detection of STEC relies heavily on enrichment; yet no standard media or protocols exist. Furthermore, whether enrichment may bias detection of multiple STEC serogroups from complex samples is unknown. Thus, 14 STEC strains of serogroups O157 and the top six non-O157s (O26, O45, O103, O111, O121, and O145) were enriched in pairs for 6–78 h in broth and evaluated by quantitative polymerase chain reaction (qPCR). Here we show that a conventional 6-h enrichment protocol did not result in intra-species culture bias for the isolates tested. However, subsequent enrichments often produced biased cultures, with differences in the qPCR gene copy number ≥2 log10 apparent in 12%, 38%, and 52% of competitions after 30, 54, and 78 h of consecutive enrichments, respectively. Some strains were able to prevail and (or) out-compete the opponent strain in 100% of competitions. Our results suggest that culture bias should be considered and (or) evaluated further due to the potential implications during routine pathogen screening and outbreak investigations.
  • OPEN ACCESS
    Altered acrylic acid concentrations in hard and soft corals exposed to deteriorating water conditions
    FACETS • Vol. 2 • pp. 531 - 544
    A reliable marker of early coral response to environmental stressors can help guide decision-making to mitigate global coral reef decline by detecting problems before the development of clinically observable disease. We document the accumulation of acrylic acid in two divergent coral taxa, stony small polyp coral (Acropora sp.) and soft coral (Lobophytum sp.), in response to deteriorating water quality characterized by moderately increased ammonia (0.25 ppm) and phosphate (0.15 ppm) concentrations and decreased calcium (360 ppm) concentration, using nuclear magnetic resonance spectroscopy (NMR)-based metabolomic techniques. Changes in acrylic acid concentration in polyp tissues free of zooxanthellae suggest that acrylic acid could be a product of animal metabolism and not exclusively a metabolic by-product of the osmolyte dimethylsulfoniopropionate (DMSP) in marine algae or bacteria. Our findings build on previously documented depletions of acrylic acid in wild coral potentially correlated to temperature stress and provide additional insight into approaches to further characterize the nature of the metabolic accumulation of acrylic acid under controlled experimental conditions.
  • OPEN ACCESS
    Knowledge sharing about deep-sea ecosystems to inform conservation and research decisions
    FACETS • Vol. 2 • pp. 984 - 997
    The Marianas Trench Marine National Monument (MNM) currently extends policy-based protection to deep-sea ecosystems contained within it, but managers require better understanding of the current knowledge and knowledge gaps about these ecosystems to guide decision-making. To address this need, we present a case study of the Marianas Trench MNM using in-depth interviews to determine scientists’ (1) current understanding of anthropogenic drivers of change and system vulnerability in deep-sea ecosystems; and (2) perceptions of the least understood deep-sea ecosystems and processes in the Marianas Trench MNM, and which of these, if any, should be research priorities to fill knowledge gaps about these systems and the impacts from anthropogenic drivers of change. Interview respondents shared similar views on the current knowledge of deep-sea ecosystems and potential anthropogenic drivers of change in the Marianas Trench MNM. Respondents also identified trench and deep pelagic (bathyal, abyssal, and hadal zones) ecosystems as the least understood, and highlighted climate change, litter and waste, mining and fishing, and interactions between these drivers of change as critical knowledge gaps. To fill key knowledge gaps and inform conservation decision-making, respondents identified the need for monitoring networks and time-series data. Our approach demonstrates how in-depth interviews can be used to elicit knowledge to inform decision-making in data-limited situations.
  • OPEN ACCESS
    Range extension for the region of sympatry between the nudibranchs Hermissenda opalescens and Hermissenda crassicornis in the northeastern Pacific
    FACETS • Vol. 3 • No. 1 • pp. 764 - 776
    The mollusc nudibranch genus Hermissenda Bergh, 1879 was recently discovered to include three pseudocryptic species, dividing a single species H. crassicornis (sensu lato) into H. crassicornis Escholtz, 1831, H. opalescens J.G. Cooper, 1863, and H. emurai Baba, 1937. The species were distinguished by both genetic and morphological evidence, and the distribution of sampled animals suggested the three species had mostly distinct geographical ranges. Here, we report the presence of both H. crassicornis and H. opalescens in Barkley and Clayoquot Sounds, British Columbia, Canada, based on diagnostic characters and molecular data congruent with the differences described for these two species. This result extends the region of sympatry for the two species from northern California, USA, to, at least, Vancouver Island, British Columbia in 2016. Depending on how long this overlap has occurred, the possible northward expansion of H. opalescens would have implications for understanding the effects of short- or long-term environmental changes in ocean temperatures as well as complicating the interpretation of past neurobiological studies of H. crassicornis (sensu lato).
  • OPEN ACCESS
    What a scientist looks like: Portraying gender in the scientific media
    FACETS • Vol. 3 • No. 1 • pp. 754 - 763
    How men and women are portrayed in the media informs societal attitudes towards gender. Although this is true for all media, the scientific media has received little scrutiny, despite known gender biases inherent in scientific culture. We asked whether the top scientific journals, Nature and Science, represented men and women equally as authors, subjects, and objects in photographs. Overwhelmingly, women were underrepresented in these magazines, an effect that was apparent even in advertisements and stock photographs. Clearly, gender bias in science exists at many levels.
  • OPEN ACCESS
    A model for training undergraduate students in collaborative science
    FACETS • Vol. 3 • No. 1 • pp. 818 - 829
    Engagement of undergraduate students in research has been demonstrated to correlate with improved academic performance and retention. Research experience confers many benefits on participants, particularly foundational skills necessary for graduate school and careers in scientific disciplines. Undergraduate curricula often do not adequately develop collaborative skills that are becoming increasingly useful in many workplaces and research settings. Here, we describe a pilot program that engages undergraduates in research and incorporates learning objectives designed to develop and enhance collaborative techniques and skills in team science that are not typical outcomes of the undergraduate research experience. We conducted a collaborative science project that engaged faculty advisors and upper year undergraduates at four institutions and conducted a review to assess the program’s efficacy. Students developed a broad suite of competencies related to collaborative science, above and beyond the experience of completing individual projects. This model also affords distinct advantages to faculty advisors, including the capacity of the network to collect and synthesize data from different regions. The model for training students to conduct collaborative science at an early stage of their career is scalable and adaptable to a wide range of fields. We provide recommendations for refining and implementing this model in other contexts.
  • OPEN ACCESS
    Can the toxicity of naphthenic acids in oil sands process-affected water be mitigated by a green photocatalytic method?
    FACETS • Vol. 5 • No. 1 • pp. 474 - 487
    Our study evaluates the efficacy of a “green” (i.e., sustainable, recyclable, and reusable) technology to treat waste waters produced by Canada’s oil sands industry. We examined the ability of a novel advanced oxidative method—ultra-violet photocatalysis over titanium dioxide (TiO2)-coated microparticles—to reduce the toxicity of naphthenic acid fraction components (NAFC) to early life stages of the fathead minnow (Pimephales promelas). Lengthening the duration of photocatalysis resulted in greater removal of NAFC from bioassay exposure waters; low- and high-intensity treatments reduced NAFC concentrations to about 20 and 3 mg/L (by Fourier-transformed infrared spectroscopy, FTIR), respectively. Treatments reduced the acute lethality of NAFC to fathead minnows by over half after low-intensity treatment and three-fold after high-intensity treatment. However, incomplete degradation in low-intensity treatments increased the incidence of chronic toxicity relative to untreated NAFC solutions and cardiovascular abnormalities were common even with >80% of NAFC degraded. Our findings demonstrate that photocatalysis over TiO2 microparticles is a promising method for mitigating the toxicity of oil sands process-affected water-derived NAFC to fish native to the oil sands region, but the intensity of the photocatalytic treatment needs to be considered carefully to ensure adequate mineralization of toxic constituents.
  • OPEN ACCESS
    An examination of the Iridovirus core genes for reconstructing Ranavirus phylogenies
    FACETS • Vol. 5 • No. 1 • pp. 523 - 533
    Ranaviruses are globally emerging infections of poikilothermic vertebrates and belong to the viral family Iridoviridae. The six species of ranaviruses are responsible for unknown numbers of infections and disease and mortality events around the world in amphibians, fish, and reptiles. Genomic investigations have shown that there are 24 core genes shared by all iridoviruses. In this study, we examine the utility of each of these genes in reconstructing phylogenetic relationships across six species of Ranavirus. We also performed dot-plot analysis for the 17 isolates in the study. For large-scale differentiation, using the major capsid protein gene creates a tree similar to the whole genome tree. Other comparable genes include open reading frame (ORF) 19R (a serine–theonine protein kinase) and ORF 88R (Erv I/Alr Family protein). The poorest candidate for phylogenetic reconstruction, due to high homology, was ORF 1R (a putative replication factor and (or) DNA binding-packing protein). There are a plethora of genes that may be useful to examine phylogenies at smaller scales (e.g., to examine local adaptation); however, they do not necessarily belong to the set of highly conserved core genes.
  • OPEN ACCESS
    Are we paying-to-play? A quantitative assessment of Canadian open access research in ecology and evolution
    FACETS • Vol. 6 • pp. 537 - 544
    Open access (OA) allows for peer-reviewed research to be freely accessed and there has been a collective shift from both researchers and publishers towards more OA publishing. OA typically occurs either through article-processing charges (the gold road) or via self-archiving (the green road); the former can be expensive, while the latter has seen minimal uptake. The gold road of OA has led to predatory publishers and, to some, questionable publications. Here, I used publicly available grant information in Canada and combined this with individual publishing statistics to test a variety of factors and their influence on OA publishing. I showed that an individual’s award amount, H-index, and gender did not influence the proportion of OA articles they published, but an individual’s H-index scaled with the number of OA publications. Institute size influenced OA publishing patterns, with researchers at large universities (i.e., >20 000 full-time students) publishing proportionately more OA articles than medium and small institutes. I discuss the potential for this pattern to build on pre-existing systemic biases when it comes to funding and publishing.
  • OPEN ACCESS
    Students’ mindsets on research integrity—a cross-cultural comparison
    FACETS • Vol. 7 • pp. 528 - 542
    Research integrity (RI) has been a focus of society in recent years as a means to create and to keep trust in science. Higher education institutions (HEIs) play a key role in promoting a culture of RI and responsible conduct of research (RCR). The understanding and practice of RI can vary across cultures. This article aims to outline initial insights into university students’ RI mindsets based on five RI facets: understanding, importance, value–action gap, enforcement approaches, and training. A qualitative exploratory cross-cultural study was conducted with participants from Germany and Bulgaria via semi-structured guided group interviews. An explicit transcultural agreement regarding the significance of RI was categorically indicated. Intercultural differences between the two European countries were revealed and discussed in reference to understanding RI, the value–action gap, enforcement approaches, and training preferences.
  • OPEN ACCESS
    Research integrity: learning from collective action in Nepal
    FACETS • Vol. 7 • pp. 236 - 246
    Early career researchers in developing countries like Nepal have faced many barriers while learning and practicing research integrity. Having easy access to appropriate resources for learning research integrity is essential to ensure academic integrity in higher education in Nepal and promote responsible research practices. This paper presents an approach to collective learning that will help stakeholders initiate learning and foster research integrity at their own level. Methodologically, the learning interventions were conducted in four phases: preparation, planning, implementation, and learning. Throughout the process of each phase, social exchange theory and collaboration in social learning were considered as new literacy models to promote research integrity knowledge. The interpretation of experiential learning interventions led to the development of the 4Co collective learning model. This model is contextually applicable for gaining deeper knowledge and skills and new networks of research integrity. With the purposes of awareness and development, this article is divided into two sections: the first part explores the actions taken and the second explores experiential learning that provides insights about the 4Co collective learning model.
  • OPEN ACCESS
    Dialogical teaching of research integrity: an overview of selected methods
    FACETS • Vol. 6 • pp. 2138 - 2154
    This communication discusses the dialogical methods of teaching research integrity and ethics as a part of the positive integrity trend focused on supporting ethical behaviour. The aim of this paper is to offer a brief overview of the selected dialogical strategies based on cases that can be successfully implemented in teaching ethical research and when sharing experiences on good scientific practice. We describe such methods as: storytelling, rotatory role playing, and the fishbowl debate, along with the “Dilemma Game” tool, “ConscienceApp” performance, and a flipped classroom idea. These theoretical considerations are based on research conducted as part of a European project under the Horizon 2020 programme.
  • OPEN ACCESS
    What’s integrity got to do with it? Second-year experiences of the Path2Integrity e-learning programme
    FACETS • Vol. 7 • pp. 110 - 125
    Organisations in Europe differ significantly in how they promote research integrity (RI). Higher education institutions play a pivotal role in disseminating a culture of RI and responsible conduct of research (RCR). Adhering and strengthening mentoring systems, implementing codes of conduct, and raising awareness are just a few initiatives among many to enhance students’ training in RCR. This article describes the Path2Integrity Learning Card (P2LIC) programme, a proactive training programme to foster RI. This programme was further developed in 2020 and the updated feedback loops took place in four countries (Germany, Denmark, Spain, and Poland). We outline the P2ILC development and final design, the trainer feedback on the programme from the second year of operation, and suggest future considerations for RCR training to strengthen research integrity.
  • OPEN ACCESS
    Introducing the Strategy for Patient Oriented Research (SPOR) Evidence Alliance: a partnership between researchers, patients and health system decision-makers to support rapid-learning and responsive health systems in Canada and beyond
    FACETS • Vol. 7 • pp. 639 - 653
    This is the introductory paper in a collection of four papers on the Strategy for Patient-Oriented Research (SPOR) Evidence Alliance, a pan-Canadian research initiative that was funded by the Canadian Institutes of Health Research in September of 2017. Here, we introduce the SPOR enterprise in Canada, provide a rationale for the creation of the SPOR Evidence Alliance, provide information on the mandate and approach, and describe how the SPOR Evidence Alliance adds to the health research ecosystem in Canada and beyond.
  • OPEN ACCESS
    An inclusive and diverse governance structure of the strategy for patient-oriented research (SPOR) Evidence Alliance
    FACETS • Vol. 7 • pp. 435 - 447
    The Strategy for Patient Oriented Research (SPOR) Evidence Alliance is a research initiative in Canada whose mission is to promote the synthesis, dissemination, and integration of research results into health care and public health decision-making and clinical practice. The aim of this paper is to (i) outline the governance and committee structure of the SPOR Evidence Alliance, (ii) outline the procedures for patient and health system decision-maker engagement, and (iii) present the capacity-building strategy for governance members. The governance structure includes the following six standing committees: the International Advisory Committee, Steering Committee, Executive Committee, Knowledge Translation Committee, Partnerships Committee, and Training and Capacity Development Committee. The guiding principles embrace inclusiveness, support, mutual respect, transparency, and co-building. There are currently 64 committee members across the six committees, 13 patient and public partners, 8 health system decision-makers, 7 research trainees, and 36 researchers. A multi-disciplinary and diverse group of people in Canada are represented from all regions and at various levels of training in knowledge generation, exchange, and translation. This collaborative model makes the SPOR Evidence Alliance strong and sustainable by leveraging the knowledge, lived experiences, expertise, skills, and networks among its 342 members and 12 principal investigators.
  • OPEN ACCESS
    A Canadian model for providing high-quality, timely and relevant evidence to meet health system decision-maker needs: the SPOR Evidence Alliance
    FACETS • Vol. 7 • pp. 420 - 434
    Canada has made great progress in synthesizing, disseminating, and integrating research findings into health systems and clinical decision-making; yet gaps exist in the research-to-practice continuum. The Strategy for Patient-Oriented Research (SPOR) Evidence Alliance aims to help close gaps by providing decision-makers with evidence that is timely, context sensitive, and demand driven to better inform patient-oriented practices and policies in health systems. In this article, we introduce a model established in Canada to support decision-maker needs for high-quality evidence that is patient oriented to enhance health systems performance. We provide an overview of how this model was implemented, who is involved, who it serves, as well as its organizational structure and remit. We discuss key milestones achieved to date and the impact this initiative has made within the health research community. The strength of the SPOR Evidence Alliance lies in its unique ability to simultaneously: (i) serve as a national platform for researchers to stay connected and collaborate to minimize duplication of efforts and (ii) facilitate access to research knowledge for patient partners and decision-makers. In doing so, the SPOR Evidence Alliance is supporting health policy and practice decisions that support and strengthen Canada’s dynamic health systems.
  • OPEN ACCESS
    Cadre législatif québécois pour la protection des espèces sauvages en situation précaire: évaluation critique et recommandations pour une révision majeure
    FACETS • Vol. 9 • pp. 1 - 14
    In Quebec, the Act Respecting Threatened or Vulnerable species (ARTV), adopted in 1989, aims to safeguard Quebec's wild genetic diversity by protecting species at risk. However, since its implementation about 30 years ago, it has been repeatedly pointed out that the application of the Quebec legislative framework for the protection of wildlife species at risk was often slow and inadequate. The aim of this article is therefore to make a series of observations on the limits of current legislation and then propose nine urgent recommendations to improve the effectiveness of conservation efforts for species at risk in Quebec. Our recommendations aim to increase the efficiency and transparency of the designation process, reconsider compensation mechanisms for the loss of critical habitat, and harmonize species status between the federal and provincial levels. We hope that our article will pave the way for a constructive discussion leading to an improved protection of wildlife species in precarious situations and their persistence for future generations. The English version of this article is available in the Supplementary material file.